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经验公式(希尔记法):
C25H22N2O5
化学文摘社编号:
分子量:
430.45
UNSPSC Code:
12171500
NACRES:
NA.47
MDL number:
产品名称
5-羧基四甲基罗丹明,
SMILES string
N(C)(C)c1cc2[o+]c3c(c(c2cc1)c4c(cc(cc4)C(=O)[O-])C(=O)O)ccc(c3)N(C)C
InChI
1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
InChI key
YMZMTOFQCVHHFB-UHFFFAOYSA-N
assay
≥95% (HPLC)
form
solid
solubility
water: slightly soluble
ε (extinction coefficient)
≥85000-97000 at 539-545 nm in methanol
fluorescence
λex ~543 nm; λem ~570 nm in methanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Application
5-羧基四甲基罗丹明已用于荧光团表征。
Biochem/physiol Actions
5-羧基四甲基罗丹明可以共价连接到 DNA 上,从而用于涉及分支和双链 DNA/RNA 结构的研究。主要用于单分子研究。5-羧基四甲基罗丹明也用于标记寡糖,用于激光诱导荧光检测。
General description
在高纯度中制备的重要荧光基团,通过约 600 个分子的毛细管电泳检测限为 TMR 标记氨基酸。容易被荧光显微镜中使用的汞弧灯的 546 nm 光谱线激发,本质上比荧光素更具光稳定性。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Macrophages are innate immune cells which can react to a large number of environmental stimuli thanks to a high degree of plasticity. These cells are involved in a variety of tissue functions in homeostasis, and they play essential roles in
G Vámosi et al.
Biophysical journal, 71(2), 972-994 (1996-08-01)
Fluorescence steady-state and lifetime experiments have been carried out on duplex and single-stranded DNA molecules labeled at the 5' ends with 5-carboxytetramethylrhodamine (TMRh). The temperature and ionic strength of the solutions were varied over large ranges. The results reveal at
Limary M Cancel et al.
Scientific reports, 8(1), 9265-9265 (2018-06-20)
We investigated the effects of direct current stimulation (DCS) on fluid and solute transport across endothelial cell (EC) monolayers in vitro. Our motivation was transcranial direct current stimulation (tDCS) that has been investigated for treatment of neuropsychiatric disorders, to enhance
Giovanni Luongo et al.
ACS applied materials & interfaces, 9(46), 40059-40069 (2017-10-13)
Iron oxide nanostructures have been widely developed for biomedical applications because of their magnetic properties and biocompatibility. In clinical applications, stabilization of these nanostructures against aggregation and nonspecific interactions is typically achieved using weakly anchored polysaccharides, with better-defined and more
High-selectivity neural probe based on a Fabry-Perot optical filter and a CMOS silicon photodiodes array at visible wavelengths
Pimenta S, et al.
Journal of Biomedical Optics, 23(10), 105004-105004 (2018)
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