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Merck
CN

D0531

5-([4,6-二氯三嗪-2-基]氨基)荧光素 盐酸盐

≥90% purity (HPLC), powder

别名:

DTAF

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关于此项目

经验公式(希尔记法):
C23H12Cl2N4O5 · HCl
化学文摘社编号:
分子量:
531.73
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
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产品名称

5-([4,6-二氯三嗪-2-基]氨基)荧光素 盐酸盐, ≥90% (HPLC)

form

powder

SMILES string

Cl.Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(Nc6nc(Cl)nc(Cl)n6)ccc45)c1

InChI

1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H

InChI key

CEKSHCAIHGNWBK-UHFFFAOYSA-N

assay

≥90% (HPLC)

color

yellow to orange

Quality Level

fluorescence

λex 495 nm; λem 516 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Application

5-([4,6-二氯三嗪-2-基]氨基)荧光素盐酸盐已用于标记酵母细胞,以研究酵母细胞对欧洲鲈鱼(Dicentrarchus labrax) 幼鱼和豹纹喙鲈(Mycteroperca rosacea)消化道的粘附。此外,它还被用于标记细菌(弧菌属
)。5-([4,6-二氯三嗪-2-基]氨基)荧光素盐酸盐已被用来共价标记黄原胶(XG)以研究其明胶行为模式。
一种荧光素衍生物,可在中性 pH 下与蛋白质的氨基和 N 末端氨基酸反应。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

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Synergistic gelation of gelatin B with xanthan gum
Wang CS, et al.
Food Hydrocolloids, 60, 374-383 (2016)
Assessment of fluorescent-labeled bacteria for evaluation of in vivo uptake of bacteria (Vibrio spp.) by crustacean larvae.
Soto-Rodriguez SA, et al.
Journal of Microbiological Methods, 52, 101-114 (2003)
Adrián Pérez-Ramos et al.
Frontiers in microbiology, 9, 1684-1684 (2018-08-10)
Lactic acid bacteria synthesize exopolysaccharides (EPS), which could benefit the host's health as immunomodulators. Furthermore, EPS could protect bacteria against gastrointestinal stress, favoring gut colonization, thus protecting the host against pathogenic infections. Pediococcus parvulus 2.6, produces a 2-substituted (1,3)-β-D-glucan and
Michael S Reid et al.
Carbohydrate polymers, 250, 116943-116943 (2020-10-15)
Fluorescently labeled cellulose nanofibrils (CNFs) were used to evaluate CNF leaching from paper according to standard safety assays for food contact materials. Enzymatically pretreated pulp was first labeled with 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF), followed by homogenization to produce fluorescent CNFs of
Effects of dietary live yeast Debaryomyces hansenii on the immune and antioxidant system in juvenile leopard grouper Mycteroperca rosacea exposed to stress.
Reyes-Becerril M, et al.
Aquaculture (Amsterdam, Netherlands), 280, 39-44 (2008)

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