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Merck
CN

D2560

DNase 测试琼脂,含甲苯胺蓝

NutriSelect® Basic, powder, for bacteria, for fungi, pack of 500 g, suitable for microbiology

别名:

DNase agar (with TBO),, DNase test agar withtoluidine blue O (TBO), Deoxyribonuclease test agar with toluidine blue

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关于此项目

NACRES:
NA.85
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Basic
Form:
powder
Suitability:
bacteria (deoxyribonuclease activity), fungi, nonselective and differential for Serratia spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp., nonselective and differential for molds (General Media), nonselective and differential for yeasts (General Media)
Application(s):
food and beverages, microbiology
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sterility

non-sterile

Quality Segment

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

color

blue

final pH

7.3±0.2 (25 °C)

application(s)

food and beverages, microbiology

suitability

bacteria (deoxyribonuclease activity), fungi, nonselective and differential for Serratia spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp., nonselective and differential for molds (General Media), nonselective and differential for yeasts (General Media)

General description

The DNase Test Agar with Toluidine Blue is a specialized microbiological medium used to detect deoxyribonuclease (DNase) activity in bacteria and fungi. It incorporates toluidine blue as an indicator, which produces a distinct color change when DNase activity is present. This medium is particularly effective for differentiating and identifying nonpigmented Serratia species. Tryptose in the formulation provides essential nutrients, while DNase activity depolymerizes DNA, triggering a bright pink reaction due to the metachromatic properties of toluidine blue. However, some Staphylococcus strains may experience growth inhibition on this medium because of the toluidine blue. Additional confirmatory tests are recommended for accurate identification.

Application

建议用于以甲苯胺蓝为指示剂检测细菌和真菌的脱氧核糖核酸酶活性。

Features and Benefits

NutriSelect® Basic is a powdered culture media offering
  • Qualitycontrol with growth promotion test according to industry standards
  • Qualityassurance acc. to ISO 9001
  • Cost-efficient powderedmedia

Preparation Note

将42.1 g含有甲苯胺蓝的DNA酶检测琼脂悬浮于1000 ml的蒸馏水中。加热并频繁搅拌至培养基完全溶解。在12至15磅压力(118°C至121°C)下高压灭菌15分钟。冷却至45°C并倾倒至培养皿中。

Other Notes

成分(g/L)
胰蛋白、20.00
脱氧核糖核酸 (DNA)、2.00
氯化钠、5.00
甲苯胺蓝、0.10
琼脂、15.00
我们现提供两种类型的培养基:GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基,满足用户不同层次的需求。
商标后的品级称号basic(基础)、plus(加强)或prime(特级)代表产品的不同质控水平,从基础的质控到标准质控加强版到全面合规的特级质控水平。

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



历史批次信息供参考:

分析证书(COA)

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