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NACRES:
NA.85
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Basic
Form:
powder
Suitability:
bacteria (deoxyribonuclease activity), fungi, nonselective and differential for Serratia spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp., nonselective and differential for molds (General Media), nonselective and differential for yeasts (General Media)
Application(s):
food and beverages, microbiology
sterility
non-sterile
Quality Segment
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
packaging
pack of 500 g
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbe id | specific enzyme detection: suitable, microbiological culture: suitable
color
blue
final pH
7.3±0.2 (25 °C)
application(s)
food and beverages, microbiology
suitability
bacteria (deoxyribonuclease activity), fungi, nonselective and differential for Serratia spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp., nonselective and differential for molds (General Media), nonselective and differential for yeasts (General Media)
General description
The DNase Test Agar with Toluidine Blue is a specialized microbiological medium used to detect deoxyribonuclease (DNase) activity in bacteria and fungi. It incorporates toluidine blue as an indicator, which produces a distinct color change when DNase activity is present. This medium is particularly effective for differentiating and identifying nonpigmented Serratia species. Tryptose in the formulation provides essential nutrients, while DNase activity depolymerizes DNA, triggering a bright pink reaction due to the metachromatic properties of toluidine blue. However, some Staphylococcus strains may experience growth inhibition on this medium because of the toluidine blue. Additional confirmatory tests are recommended for accurate identification.
Application
建议用于以甲苯胺蓝为指示剂检测细菌和真菌的脱氧核糖核酸酶活性。
Features and Benefits
NutriSelect® Basic is a powdered culture media offering
- Qualitycontrol with growth promotion test according to industry standards
- Qualityassurance acc. to ISO 9001
- Cost-efficient powderedmedia
Preparation Note
将42.1 g含有甲苯胺蓝的DNA酶检测琼脂悬浮于1000 ml的蒸馏水中。加热并频繁搅拌至培养基完全溶解。在12至15磅压力(118°C至121°C)下高压灭菌15分钟。冷却至45°C并倾倒至培养皿中。
Other Notes
成分(g/L)
胰蛋白、20.00
脱氧核糖核酸 (DNA)、2.00
氯化钠、5.00
甲苯胺蓝、0.10
琼脂、15.00
胰蛋白、20.00
脱氧核糖核酸 (DNA)、2.00
氯化钠、5.00
甲苯胺蓝、0.10
琼脂、15.00
我们现提供两种类型的培养基:GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基,满足用户不同层次的需求。
商标后的品级称号basic(基础)、plus(加强)或prime(特级)代表产品的不同质控水平,从基础的质控到标准质控加强版到全面合规的特级质控水平。
商标后的品级称号basic(基础)、plus(加强)或prime(特级)代表产品的不同质控水平,从基础的质控到标准质控加强版到全面合规的特级质控水平。
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)