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Merck
CN

D9015

3,3-二氨基联苯胺 四盐酸盐 水合物

≥96% purity (TLC), powder

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关于此项目

线性分子式:
C12H14N4 · 4HCl · xH2O
化学文摘社编号:
868272-85-9
NACRES:
NA.47
PubChem Substance ID:
24894241
UNSPSC Code:
12171500
EC Number:
231-018-9
MDL number:
MFCD08273058

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产品名称

3,3-二氨基联苯胺 四盐酸盐 水合物, ISOPAC®

InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

SMILES string

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

assay

≥96% (TLC)

form

powder

color

off-white to faint red, to beige

mp

280 °C

solubility

water: 50 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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相关类别

Application

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

Disclaimer

Injecting a compatible solvent permits preparation of any desired strength solution without exposure.

Packaging

Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.

Preparation Note

Dissolving the contents in 100 mL of solvent yields a 0.1% solution.

Legal Information

Isopac is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCW2098
MKCQ1964
MKCK6532
MKCC3553
MKBT9193V

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Mammalian AMP-activated protein kinase subfamily.
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The Journal of Biological Chemistry, 271, 611-614 (1996)
A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor.
Herzog V and Fahimi HD
Analytical Biochemistry, 55, 554-562 (1973)
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Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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