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Merck
CN

E1903

乙锭同型二聚体 I 溶液

≥95% purity (HPLC), liquid

别名:

EtDi, EthD-1, 菲啶，5,5′-- [1,2-乙二基双（亚氨基-3,1-丙二基）] 双（3,8-二氨基-6-苯基）-, 二氯化物，二盐酸盐

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关于此项目

经验公式（希尔记法）:
C46H50Cl4N8
分子量:
856.75
NACRES:
NA.47
PubChem Substance ID:
24894444
UNSPSC Code:
12171500
EC Number:
200-664-3
MDL number:
MFCD00210948

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产品名称

乙锭同型二聚体 I 溶液, ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO

InChI key

ZKAJUKABUUJRLB-UHFFFAOYSA-N

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

SMILES string

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

assay

≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)

form

liquid

concentration

2 mM in DMSO

technique(s)

titration: suitable

color

red

ε (extinction coefficient)

≥9000 at 490-496 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

100

相关类别

Application

已使用乙锭同型二聚体 I 溶液检查精子中的膜完整性。
染色 ssDNA、dsDNA、RNA、寡核苷酸和三链 DNA 结构。
用于检测溶液中的 DNA。

Biochem/physiol Actions

1 分子 EthD-1 将非选择性结合 dsDNA 中的 4 个碱基对。
乙锭同型二聚体是一种红色荧光染料。它不能穿过完整的质膜，但很容易通过受损的质膜。使用乙锭同型二聚体检查膜完整性。

存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

185.0 °F - closed cup

flash_point_c

85 °C - closed cup

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCX9687
MKCX4268
MKCV5389
MKCV8458
MKCT2329

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C Carlsson et al.
Nucleic acids research, 23(13), 2413-2420 (1995-07-11)
Many bis-intercalating dyes used for fluorescence detection of DNA in electrophoresis have been reported to give band-splitting and band-broadening, which results in poor resolution and a decreased detection sensitivity. We have studied the dimeric dye YOYO-1, and to some extent
Desiccation tolerance in bovine sperm: a study of the effect of intracellular sugars and the supplemental roles of an antioxidant and a chelator.
Sitaula R, et al.
Cryobiology, 58, 322-330 (2009)
E Tuite et al.
Bioorganic & medicinal chemistry, 3(6), 701-711 (1995-06-01)
The binding of phenanthridine dyes to triplex poly(dT)*poly(dA).poly(dT) and its precursor duplex poly(dA).poly(dT) is characterized using linear dichroism and circular dichroism spectroscopy, and thermal denaturation. The two monomeric dyes ethidium bromide and propidium iodide are shown to behave similarly to
Samuel B Tristan-Landin et al.
PloS one, 14(10), e0215114-e0215114 (2019-10-11)
Fluorescence microscopy is one of the workhorses of biomedical research and laboratory diagnosis; however, their cost, size, maintenance, and fragility has prevented their adoption in developing countries or low-resource settings. Although significant advances have decreased their size, cost and accessibility

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