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E7889

乙二胺四乙酸 二钠盐 溶液

Molecular Biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

别名:

EDTA 二钠盐, EDTA-Na2, 亚乙基二次氮基四乙酸 二钠盐

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关于此项目

线性分子式:
[-CH2N(CH2CO2Na)CH2CO2H]2
化学文摘社编号:
139-33-3
分子量:
336.21
UNSPSC Code:
12352107
NACRES:
NA.31
PubChem Substance ID:
24894660
MDL number:
MFCD00070672
Beilstein/REAXYS Number:
3822669
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grade

Molecular Biology

Quality Level

300

sterility

0.2 μm filtered

form

liquid

reaction suitability

reagent type: chelator

concentration

0.5 M in H2O

pH

7.5-8.5

suitability

suitable for molecular biology

application(s)

microbiology

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

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General description

乙二胺四乙酸二钠盐溶液是一种螯合剂，适用于分子生物学应用。
乙二胺四乙酸（EDTA）是一种多元酸。EDTA中带孤对电子的四个羧酸和两个胺基可以螯合钙离子及其他几种金属离子。

Application

乙二胺四乙酸二钠盐溶液已应用于：
  • 盐酸（HCL）中消化蛋白酶-K，以测定角膜样本中DNA的含量
  • 与木瓜蛋白酶结合消化角膜样本，以量化糖胺聚糖（GAG）的含量
  • 预处理中，以剥离生长至80%融合的上皮细胞
  • 制备裂解缓冲液的原液
  • 制备尿素裂解缓冲液

Features and Benefits

分子生物学级

Preparation Note

用分子生物学试剂 EDTA (E 5134) 在 18 兆欧水中制备

Other Notes

如需了解生化试剂系列的更多信息，请填写此表。

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H373

Hazard Classifications

STOT RE 2 Inhalation

target_organs

Respiratory Tract

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000514196
0000514194
0000431452
0000428555
0000426649

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实验方案

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

全球贸易项目编号

货号GTIN
E7889-100ML04061838255334
E7889-1L04061838110152

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