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安全信息

H3149

Sigma-Aldrich

肝素 钠盐 来源于猪肠粘膜

Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture

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CAS号:
9041-08-1
MDL编号:
MFCD00081689
NACRES:
NA.21

生物来源

Porcine intestinal mucosa

质量水平

200

类型

Grade I-A

产品线

BioReagent

形式

powder

比活

≥180 USP units/mg

技术

analytical sample preparation: suitable
cell culture | mammalian: suitable

颜色

beige

溶解性

H2O: 50 mg/mL

应用

cell analysis
diagnostic assay manufacturing
life science and biopharma
sample preparation

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相关类别

一般描述

肝素主要用于推迟血液的凝固。它在凝血途径中可增强抗凝血酶介导的蛋白酶失活。
肝素钠是一种来自糖胺聚糖家族的硫酸化多糖，在细胞培养、癌症生物学和药物发现研究中发挥着至关重要的作用。它对蛋白质和细胞相互作用的影响使其对于研究分子和细胞过程、肿瘤建模、探索结合相互作用以及作为支持细胞活力的细胞培养补充剂很有价值。

应用

  • 来自猪肠粘膜的肝素钠盐已用于从人血浆中纯化sLRP（可溶性循环低密度脂蛋白受体相关蛋白-1）。
  • 它已被用作用于培养人脐静脉内皮细胞的M199培养基中的一种补充剂。
  • 它在血液分离的过程中被用作抗凝血剂。

特点和优势

  • 高纯度化合物适用于各种研究应用

其他说明

为了全面了解我们针对客户研究提供的各种多糖产品，建议您访问我们的碳水化合物分类页面。
For additional information on our range of Biochemicals, please complete this form.

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品

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  1. How is Heparin sodium salt from porcine intestinal mucosa, Product H3149, solubilized?

    Heparin sodium salt from porcine intestinal mucosa is soluble in deionized water at a concentrationi of 50 mg/mL.

  2. How much of Heparin sodium salt from porcine intestinal mucosa, Product H3149, is used for cell culture?

    Solutions at 0.5 mg per mL were tested in cell culture. Start at this concentration, and titer from there to determine the optimal concentration for usage.

  3. How are solutions Heparin sodium salt from porcine intestinal mucosa, Product H3149, stored?

    Solutions can be stored at 2-8C for up to 2 years, if sterile filtered through a 0.22 micron filter. Benzyl alcohol (0.06-1%) can be added to solutions to prevent microbial growth.

  4. How many milligrams of Heparin sodium salt, Product H3149, are in the bottle?

    The potency, in units/mg, is lot-specific; the value can be found on the certificate of analysis. It is typically over 140 units per mg. The number of milligrams can be calculated by dividing the number of units by the potency in units per mg.

  5. What is the molecular weight of Heparin sodium salt?

    Heparin is a mixture of polyanion chains having molecular weights ranging from 6,000 to 30,000 Daltons, with most chains in the range of 17,000 to 19,000 Daltons.

  6. How much Heparin sodium salt, is used to prevent coagulation?

    We suggest using 20 to 50 units per mL of blood.

  7. What is the best way to sterilize Heparin sodium salt?

    Although solutions may be autoclaved at 121 °C for 5 to 10 minutes, we generally recommend sterile filtration. This is because prolonged autoclaving may have an effect on its activity.  

  8. What concentration of Heparin sodium salt, can I use to inhibit RNAse activity?

    Heparin has been used at concentrations ranging from 100 to 500 μg/mL. This has been reported in the J. Biol. Chem., 248, 2095 (1973).

  9. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  10. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  11. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  12. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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