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I6758

IPTG

Name: IPTG
Brand: SIAL

≥99% (TLC), ≤0.1% Dioxane

别名:

异丙基β-D-1-硫代吡喃半乳糖苷, IPTG, 异丙基β-D-硫代半乳糖苷

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关于此项目

经验公式（希尔记法）:

C₉H₁₈O₅S

化学文摘社编号:

367-93-1

分子量:

238.30

UNSPSC Code:

12352201

NACRES:

NA.31

PubChem Substance ID:

24896093

EC Number:

206-703-0

Beilstein/REAXYS Number:

4631

MDL number:

MFCD00063273

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biological source

synthetic

Quality Level

200

description

non-ionic

assay

≥99% (TLC)

form

powder

technique(s)

thin layer chromatography (TLC): suitable

impurities

≤0.1% Dioxane

color

white to off-white

useful pH range

5-7 (50 g/L)

mp

105 °C ((221 °F))

solubility

water: 0.05 g/L, clear

application(s)

cell analysis
life science and biopharma

storage temp.

2-8°C

SMILES string

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

General description

异丙基 β-D-硫代半乳糖苷 (IPTG) 是一种分子生物学试剂，用于诱导细菌系统中的基因表达。它模仿乳糖，与 lac 阻遏蛋白结合，导致基因激活。IPTG 对于研究基因调控、蛋白质表达和重组蛋白质生产至关重要，为细胞和分子生物学研究提供了宝贵的见解。

Application

IPTG 可用于：

诱导罗伊氏乳杆菌 GroEL 和突变体中的蛋白质表达
在大肠杆菌中诱导蛋白质表达

IPTG通常用于需要诱导β-半乳糖苷酶活性的克隆程序。它与 X-Gal 或 Bluo-Gal 一起用于重组细菌菌落的蓝白斑筛选，其诱导 lac 操纵子在大肠杆菌中的表达。IPTG 通过与 lacI 阻遏物结合，并改变其构象起作用，其阻止 β-半乳糖苷酶编码基因 lacZ 的阻遏作用。

Biochem/physiol Actions

IPTG 是异乳糖的不可代谢类似物。它作为诱导子调节 lac 启动子的转录活性，该启动子用于在大肠杆菌中表达该蛋白质。

Preparation Note

将 IPTG 溶于水，然后经无菌过滤，配制成储存液 (0.1M)。指示板中 IPTG 最终浓度应为 0.2mM。

Other Notes

为了全面了解我们针对客户研究提供的各种单糖产品，建议您访问我们的碳水化合物分类页面。

如需了解生化试剂系列的更多信息，请填写此表。

非代谢性半乳糖类似物。

pictograms

GHS08

signalword

Danger

hcodes

H350

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 1B

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Lactobacillus stress protein GroEL prevents colonic inflammation.

Alexandre M M Dias et al.

Journal of gastroenterology, 56(5), 442-455 (2021-03-31)

We previously showed that supernatants of Lactobacillus biofilms induced an anti-inflammatory response by affecting the secretion of macrophage-derived cytokines, which was abrogated upon immunodepletion of the stress protein GroEL. We purified GroEL from L. reuteri and analysed its anti-inflammatory properties

A self-inducible heterologous protein expression system in Escherichia coli

Briand L, et al.

Scientific Reports, 6 , 33037 -33037 (2016)

Serum amyloid A1 exacerbates hepatic steatosis via TLR4-mediated NF-κB signaling pathway.

Bin Jiang et al.

Molecular metabolism, 59, 101462-101462 (2022-03-06)

Chronic inflammatory response plays a prominent role in obesity-related nonalcoholic fatty liver disease (NAFLD). However, the intrahepatic triggering mechanism of inflammation remains obscure. This study aimed to elucidate the role of serum amyloid A1 (SAA1), an acute-phase response protein, in

Bimetallic gold-silver nanoparticles mediate bacterial killing by disrupting the actin cytoskeleton MreB.

Prajna Jena et al.

Nanoscale, 12(6), 3731-3749 (2020-01-30)

The actin cytoskeleton is required for the maintenance of the cell shape and viability of bacteria. It remains unknown to which extent nanoparticles (NPs) can orchestrate the mechanical instability by disrupting the cytoskeletal network in bacterial cells. Our work demonstrates

Analysis of papaya cell wall-related genes during fruit ripening indicates a central role of polygalacturonases during pulp softening.

João Paulo Fabi et al.

PloS one, 9(8), e105685-e105685 (2014-08-28)

Papaya (Carica papaya L.) is a climacteric fleshy fruit that undergoes dramatic changes during ripening, most noticeably a severe pulp softening. However, little is known regarding the genetics of the cell wall metabolism in papayas. The present work describes the

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全球贸易项目编号

货号	GTIN
I6758-10G	04061835367061
I6758-5G	04061835367085
I6758-1G	04061835367078

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