产品名称
Middlebrook 7H10 琼脂基础, NutriSelect® Plus, powder, for mycobacteria, pack of 500 g
sterility
non-sterile
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
packaging
pack of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
final pH
6.6±0.2 (25 °C)
application(s)
clinical testing
food and beverages
life science and biopharma
microbiology
veterinary
suitability
mycobacteria
Quality Level
Other Notes
成分 (g/L)
硫酸铵，0.50
L-谷氨酸，0.50
磷酸二氢钾，1.50
磷酸二钠，1.50
柠檬酸钠，0.40
柠檬酸铁铵，0.04
硫酸镁，0.025
氯化钙，0.0005
硫酸锌，0.001
硫酸铜，0.001
盐酸吡多辛，0.001
生物素，0.0005
孔雀绿，0.00025
琼脂，15.00
硫酸铵，0.50
L-谷氨酸，0.50
磷酸二氢钾，1.50
磷酸二钠，1.50
柠檬酸钠，0.40
柠檬酸铁铵，0.04
硫酸镁，0.025
氯化钙，0.0005
硫酸锌，0.001
硫酸铜，0.001
盐酸吡多辛，0.001
生物素，0.0005
孔雀绿，0.00025
琼脂，15.00
我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。
商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。
商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。
Application
Middlebrook 7H10琼脂培养基是结核分枝杆菌分离、培养和敏感性试验的选择性培养基。分枝杆菌在肉汤培养基中生长最快，因此可以进行细菌种类的初步分离。它经济、易于制备，可以帮助早期检测抗酸性，培养分枝杆菌种类。
General description
Middlebrook 7H10琼脂培养基推荐用于结核分枝杆菌的分离、培养和敏感性试验。Middlebrook培养基由许多无机盐组成，有助于分枝杆菌的生长。由柠檬酸钠形成的柠檬酸有助于保留溶液中的无机阳离子。甘油可提供碳和能量。Middlebrook OADC生长补充剂（M0678）含有油酸、牛白蛋白、氯化钠、葡萄糖和过氧化氢酶。油酸和其他长链脂肪酸是分枝杆菌代谢所必需的。一些游离脂肪酸对分枝杆菌是有毒的，但白蛋白与这些脂肪酸结合后，防止了对分枝杆菌的毒性作用。葡萄糖是一种能量来源。过氧化氢酶中和有毒过氧化物。孔雀石绿部分抑制其他细菌。
Preparation Note
将19.47 g Middlebrook 7H10 Agar Base 悬浮 900 ml蒸馏水中。加入5 ml甘油（Cat.No. 49767).加热至沸腾以完全溶解培养基。以180 ml的量分配至每个锥形瓶，并在121°C，15 磅的压力下高压灭菌10分钟。冷却至45°C，无菌添加20 ml的 Middlebrook OADC 生长补充剂（Cat.No.M0678）。充分混合，倒入带螺旋盖的试管。注意：制备好的培养基，在接种前、后均需放置在暗处。
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
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Bailey and Scott's Diagnostic Microbiology (1990)
A biocide-free mineral oil nanoemulsion exhibiting strong bactericidal activity against Mycobacterium immunogenum and Pseudomonas aeruginosa
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Ssengooba W, et al.
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Ronald J Rieder et al.
Antimicrobial agents and chemotherapy, 53(11), 4598-4603 (2009-08-26)
Methods currently used for in vitro drug susceptibility testing are based on the assessment of bacterial growth-related processes. This reliance on cellular reproduction leads to prolonged incubation times, particularly for slowly growing organisms such as mycobacteria. A new rapid phenotypic
商品
Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.
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