N2125
萘酚 AS-BI 磷酸盐
≥98% purity (HPLC), powder
别名:
7-溴-3-羟基-2-萘-o-茴香醚磷酸盐
Product Name
萘酚 AS-BI 磷酸盐, Technical grade, ≥98% (HPLC)
质量水平
方案
≥98% (HPLC)
表单
powder
技术
microbe id | staining: suitable
颜色
white to light yellow
溶解性
methanol: 50 mg/mL
应用
diagnostic assay manufacturing
hematology
histology
储存温度
−20°C
SMILES字符串
COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O
InChI
1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
InChI key
HUXIAXQSTATULQ-UHFFFAOYSA-N
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一般描述
色酚AS-BI（也称为7-溴代-3- 羟基-2-萘酚-o-茴香胺）是一种取代萘酚。
酸性和碱性磷酸酶组织化学显示的底物。
应用
萘酚AS-BI磷酸可用于：
- 抗酒石酸酸性磷酸酶（TRAP）染色，以检测破骨细胞活性
- Tris-HCl染色细胞的组成部分，以测定碱性磷酸酶（ALP）的活性
- 进行碱性磷酸酶反应
酸性和碱性磷酸酶组织化学显示的底物。 酸性和碱性磷酸酶荧光测定法的底物。石蜡包埋组织中酸性磷酸酶的证明。单个活细胞中酸性磷酸酶的细胞荧光测定法。
生化/生理作用
色酚AS-BI可用作测定碱性和酸性磷酸酶的底物。也可用于测定单细胞酸性磷酸酶的活性。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
Z Szegedi et al.
Pathology oncology research : POR, 4(3), 217-223 (1998-10-08)
The aim of the study was to work out a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis
W M Frederiks et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35(2), 175-180 (1987-02-01)
Acid phosphatase activity has been demonstrated in rat liver with the semipermeable membrane technique using naphthol AS-BI phosphate as substrate and hexazotized pararosaniline (HPRA) as simultaneous coupling agent. With this method the final reaction product (FRP) appeared in rat liver
Anthony J Janckila et al.
Clinica chimica acta; international journal of clinical chemistry, 347(1-2), 157-167 (2004-08-18)
Our purpose was to develop a specific immunoassay for tartrate-resistant acid phosphatase (TRACP) 5b using naphthol ASBI phosphate (N-ASBI-P) as a selective substrate for isoform 5b and heparin as a selective inhibitor of isoform 5a. Serum TRACP 5a and 5b
A M Boesen
Scandinavian journal of haematology, 32(3), 245-252 (1984-03-01)
The subcellular localization of acid phosphatase (AcP) in various immunologically-defined neoplastic lymphoid cells including hairy cells was investigated by electron microscopy. 2 substrates, naphthol-AS-BI-phosphoric acid (naphthol-AS-BI-P) and sodium beta-glycerophosphate, were compared. By incubation in naphthol-AS-BI-P containing medium, the reaction product
Naphthol-ASBI phosphate as a preferred substrate for tartrate-resistant acid phosphatase isoform 5b.
A J Janckila et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 16(4), 788-793 (2001-04-24)
Tartrate-resistant acid phosphatase (TRAP) isoform 5b is a potential serum marker for osteoclastic activity. Biochemical assays for serum TRAP activity with para-nitrophenylphosphate (pNPP) have low specificity for bone because of hydrolysis by unrelated nontype 5 TRAPs of blood cells and
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