N2125
萘酚 AS-BI 磷酸盐
≥98% purity (HPLC), powder
别名:
7-溴-3-羟基-2-萘-o-茴香醚磷酸盐
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关于此项目
经验公式（希尔记法）:
C18H15BrNO6P
化学文摘社编号:
分子量:
452.19
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
217-645-0
MDL number:
Beilstein/REAXYS Number:
6668526
产品名称
萘酚 AS-BI 磷酸盐, Technical grade, ≥98% (HPLC)
InChI key
HUXIAXQSTATULQ-UHFFFAOYSA-N
InChI
1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
SMILES string
COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O
assay
≥98% (HPLC)
form
powder
technique(s)
microbe id | staining: suitable
color
white to light yellow
solubility
methanol: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
Quality Level
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Application
萘酚AS-BI磷酸可用于：
- 抗酒石酸酸性磷酸酶（TRAP）染色，以检测破骨细胞活性
- Tris-HCl染色细胞的组成部分，以测定碱性磷酸酶（ALP）的活性
- 进行碱性磷酸酶反应
酸性和碱性磷酸酶组织化学显示的底物。 酸性和碱性磷酸酶荧光测定法的底物。石蜡包埋组织中酸性磷酸酶的证明。单个活细胞中酸性磷酸酶的细胞荧光测定法。
Biochem/physiol Actions
色酚AS-BI可用作测定碱性和酸性磷酸酶的底物。也可用于测定单细胞酸性磷酸酶的活性。
General description
色酚AS-BI（也称为7-溴代-3- 羟基-2-萘酚-o-茴香胺）是一种取代萘酚。
酸性和碱性磷酸酶组织化学显示的底物。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
W M Frederiks et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35(2), 175-180 (1987-02-01)
Acid phosphatase activity has been demonstrated in rat liver with the semipermeable membrane technique using naphthol AS-BI phosphate as substrate and hexazotized pararosaniline (HPRA) as simultaneous coupling agent. With this method the final reaction product (FRP) appeared in rat liver
Z Szegedi et al.
Pathology oncology research : POR, 4(3), 217-223 (1998-10-08)
The aim of the study was to work out a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis
The presence of a low molecular weight acid phosphatase in liver tissue that cannot be demonstrated with the histochemical substrate naphthol AS-BI phosphate.
A E Meijer et al.
Histochemistry, 67(1), 23-29 (1980-01-01)
Naphthol-ASBI phosphate as a preferred substrate for tartrate-resistant acid phosphatase isoform 5b.
A J Janckila et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 16(4), 788-793 (2001-04-24)
Tartrate-resistant acid phosphatase (TRAP) isoform 5b is a potential serum marker for osteoclastic activity. Biochemical assays for serum TRAP activity with para-nitrophenylphosphate (pNPP) have low specificity for bone because of hydrolysis by unrelated nontype 5 TRAPs of blood cells and
M Kimura et al.
Histochemistry, 82(6), 519-523 (1985-01-01)
A cytochemical study of acid phosphatase (AcP-ase) in the lateral prostate of the rat was performed to investigate whether AcP-ase in the secretory apparatus can be distinguished from AcP-ase in lysosomes and their related structures. Two types of AcP-ase were
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