O1875
蓄黑酚 一水合物
≥99% purity (HPLC), powder
别名:
3,5-二羟基甲苯, 5-甲基间苯二酚
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关于此项目
线性分子式:
CH3C6H3-1,3-(OH)2 · H2O
化学文摘社编号:
分子量:
142.15
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
207-984-2
MDL number:
Beilstein/REAXYS Number:
1071903
产品名称
蓄黑酚 一水合物, colorimetric detection reagent
InChI key
NBKPNAMTHBIMLA-UHFFFAOYSA-N
InChI
1S/C7H8O2.H2O/c1-5-2-6(8)4-7(9)3-5;/h2-4,8-9H,1H3;1H2
SMILES string
O.Cc1cc(O)cc(O)c1
biological source
synthetic
assay
≥99% (HPLC)
form
powder
technique(s)
titration: suitable
color
white to light brown
bp
290 °C (lit.)
mp
56-58 °C (lit.)
solubility
H2O: 50 mg/mL
density
1.290 g/cm3
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
General description
地衣酚(Orcinol)是一种小分子天然酚类物质，可制备地衣红(Orcein)染料，用于细胞和染色体染色。地衣酚一水合物常用于制备比色试剂Bial试剂，用于测定RNA、DNA和己糖。
Application
苔黑酚一水合物已用于制备 Bials 试剂（Bial′s reagen），以便Bials苔黑酚法(Bial′s orcinol assay)测定戊糖。它还可用于制备苔黑酚溶液，适于TLC板染色和苔黑酚法染料测定法鼠李糖脂测定。苔黑酚一水合物已用于 RNA 分析。
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Marjan De Mey et al.
Analytical biochemistry, 353(2), 198-203 (2006-03-21)
Recent developments in cellular and molecular biology require the accurate quantification of DNA and RNA in large numbers of samples at a sensitivity that enables determination on small quantities. In this study, five current methods for nucleic acid quantification were
Lipopolysaccharide binding protein expression in primary human hepatocytes and HepG2 hepatoma cells.
Grube BJ
The Journal of Biological Chemistry, 269, 8477-8477 (1994)
A simultaneous estimation method of DNA and RNA by the orcinol reaction and a study on the reaction mechanism.
Y Endo
Journal of biochemistry, 67(5), 629-633 (1970-05-01)
Natural products as fungicide and their role in crop protection. et al.
Natural Antimicrobials for the Minimal Processing of Foods, 131-131 (2020)
Rapid colorimetric assays to qualitatively distinguish RNA and DNA in biomolecular samples.
Patterson Jennifer and Cameron Mura
Journal of Visualized Experiments (2013)
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