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Merck
CN

P7170

丽春红S染液(零售包装) 溶液

BioReagent, 0.1 % (w/v) in 5% acetic acid

别名:

Ponceau S

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关于此项目

经验公式(希尔记法):
C22H12N4Na4O13S4
化学文摘社编号:
分子量:
760.57
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
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产品名称

丽春红S染液(零售包装) 溶液, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

description

for protein analysis

product line

BioReagent

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

technique(s)

western blot: suitable

density

1.01 g/cm3 at 20 °C

suitability

suitable (for use in cellulose acetate electrophoresis), suitable for electrophoresis

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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General description

丽春红S是重氮染料的钠盐。丽春红S溶液是一种适用于电泳研究的试剂。丽春红S色剂是现成的,专为快速(5分钟)染色硝化纤维或PVDF膜(蛋白质印记分析)上的蛋白条带,也染色醋酸纤维素膜上的蛋白质。它可作为纯化的纤溶酶(CK)的染色剂,利用蛋白测序仪对CK前14个残基的N-端氨基酸序列进行分析。硝化纤维和PVDF上的丽春红染色是可逆的,可通过0.1M NaOH冲洗染色膜1分钟脱色。染色和脱色后的膜可用于免疫检测(蛋白印迹分析)。所提供的产品信息表中包括醋酸纤维素、硝基纤维素和PVDF的染色程序。

Application

丽春红S溶液已可用于膜染色(蛋白印迹分析)。

存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

为满足蛋白分析的多样化需求,Sigma提供多种蛋白质可视化(染色)试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计,即使在传统的PAGE形式中依然表现出色。

实验方案

Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.

带有用于不同印迹过程工作流程步骤的Western blot实验方法,描述了蛋白从SDS聚丙烯酰胺凝胶(SDS-PAGE)到硝酸纤维素膜(NC膜)的电泳转移。也称为免疫印迹实验方法。

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