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10771

Histopaque®-1077

sterile-filtered, density: 1.077

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NACRES:
NA.75
UNSPSC Code:
12352207
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sterility

sterile-filtered

Quality Level

400

form

liquid

shelf life

Expiry date on the label.

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

density

1.077

application(s)

hematology
histology

storage temp.

2-8°C

相关类别

General description

Histopaque-1077 是一种无菌、经过内毒素测试的即用型培养基。Histopaque组合液包括聚蔗糖和泛影酸钠，密度经调节后为1.077g/mL。这种即用型试剂有助于从小体积的全血或骨髓中进行活性淋巴细胞和其他单个核细胞的快速恢复。

Application

Histopaque-1077用于：
  • 人淋巴细胞抗原(HLA)分型
  • T、B和‘裸’淋巴细胞计数前的起始分离。
  • 制备用于细胞培养和细胞毒性试验的纯淋巴细胞悬液。
  • 分离和纯化外周血单个核细胞(PBMC)和骨髓单个核细胞(BMMC)
  • 分离人类淋巴细胞
  • 分离全血中乳癌循环肿瘤细胞(CTC)
  • Histopaque-1077适合与Histopaque-1119一起使用，因为其有助于从少量全血中快速恢复活性单个核细胞和粒细胞

Biochem/physiol Actions

将抗凝血铺在Histopaque-1077液面上。离心时，红细胞被聚蔗糖聚集并迅速沉淀。粒细胞变得略微高渗，它们的沉降速率增加，在ACCUSIN管底部形成沉淀。淋巴细胞和其他单个核细胞留在血浆/Histopaque-1077分界面。

Features and Benefits

Histopaque®-1077 有许多优势，比如：
  • 可使活细胞极佳恢复
  • 允许选择分离不同种类的血细胞
  • 防止细胞变形
  • 保持细胞活力
  • 最小化外来细胞干扰
  • 各批次性能可重复
  • 经过过滤杀菌和内毒素检测
  • 在 2-8°C 避光保护条件下可稳定保存至少2年

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM1878
RNBM0545
RNBM0063
RNBL7683
RNBM0064

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Erin E Thacker et al.
Vaccine, 27(50), 7116-7124 (2009-09-30)
Targeting viral vectors encoding tumor-associated antigens to dendritic cells (DCs) in vivo is likely to enhance the effectiveness of immunotherapeutic cancer vaccines. We have previously shown that genetic modification of adenovirus (Ad) 5 to incorporate CD40 ligand (CD40L) rather than
Evaluation of respiration with clark type electrode in isolated mitochondria and permeabilized animal cells.
Silva AM and Oliveira PJ
Methods in Molecular Biology, 810, 7-24 (2012)
Romain A Colas et al.
Journal of immunology (Baltimore, Md. : 1950), 197(11), 4444-4452 (2016-11-02)
Maresin 1 (MaR1) is an immunoresolvent that governs resolution of acute inflammation, and its local metabolism in the context of infectious inflammation is of interest. In this study, we investigated the MaR1 metabolome in infectious exudates and its bioactions in
Maria Björkqvist et al.
The Journal of experimental medicine, 205(8), 1869-1877 (2008-07-16)
Huntington's disease (HD) is an inherited neurodegenerative disorder characterized by both neurological and systemic abnormalities. We examined the peripheral immune system and found widespread evidence of innate immune activation detectable in plasma throughout the course of HD. Interleukin 6 levels
Ana C Leal et al.
Scientific reports, 7(1), 6438-6438 (2017-07-27)
Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release of tumor-derived extracellular vesicles and the activation of host vascular cells. It was proposed that neutrophil extracellular
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商品

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

默克密度梯度介质_细胞分离-默克生命科学

我们提供完整的密度梯度介质产品线，用于分离或提取白细胞、病毒、DNA、RNA、细胞器以及许多其他应用。我们的产品线包括用于在研究实验室中分离白细胞的Histopaque®碘化梯度介质，用于哺乳动物细胞分离的多糖介质，用于哺乳动物细胞和细胞器分离的胶体二氧化硅介质，以及用于DNA、病毒和蛋白质分离的无机盐介质。

实验方案

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

Histopaque® 密度梯度介质故障排除指南

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。

全球贸易项目编号

货号GTIN
10771-10L04061837279089
10771-6X100ML04061838260642
10771-100ML04061838260628
10771-500ML04061838260635
10771-1GA04061837279096

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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