Merck
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文件

10831

Sigma-Aldrich

Histopaque®-1083

sterile-filtered, density: 1.083 g/mL

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NACRES:
NA.75

无菌性

sterile-filtered

质量水平

400

保质期

Expiry date on the label.

杂质

endotoxin, tested

密度

1.083 g/mL

application(s)

hematology
histology

储存温度

2-8°C

相关类别

一般描述

Histopaque®-1083是一种含有聚蔗糖和泛影酸钠的溶液，密度调整至-1083 g/mL。它有助于从大鼠、小鼠和其他小型哺乳动物的少量血液中分离活的单核细胞。

应用

Histopaque®-1083用于从大鼠外周血中分离单核细胞和白细胞。

特点和优势

Histopaque®-1083 有许多优势，比如：

  • 可使活细胞极佳恢复
  • 允许选择分离不同种类的血细胞
  • 防止细胞变形
  • 保持细胞活力
  • 最小化外来细胞干扰
  • 各批次性能可重复
  • 经过过滤杀菌和内毒素检测
  • 在 2-8°C 避光保护条件下可稳定保存至少2年

原理

将抗凝血液铺在HISTOPAQUE1083上。离心时，红细胞被聚多糖聚集并迅速沉淀，而淋巴细胞和其他单核细胞保留在血浆-HISTOPAQUE1083 界面。红细胞会沉淀到离心管的底部。

法律信息

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Corrosion
GHS05

警示用语：

Warning

危险声明

H290 - H315 - H319

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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示例

T1503
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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are there limitations when attempting to use Product 10831, Histopaque®-1083 with the various Accuspin products?

    Yes.  There are some limitations.  Small amounts of blood cannot be processed in the Accuspin tubes.  At least 3 mls of whole peripheral blood is expected.  Without sufficient red cell numbers, the mononuclear band fails to adequately clear the barrier or frit found within the Accuspin tubes.

  6. I find mononuclear cells separate well from human blood over Product 10831, Histopaque®-1083, but the cell recovery from rats and mice is lower.  Why?

    The most common cause for separation problems with Histopaque® 1083 is the blood source.  Blood from rats and mice is harder to obtain than blood from a human.  Most users experiencing problems are collecting blood with a syringe.  It is relatively uncommon for users to call in about poor separation when using vacuum tubes.  Humanely sacrifice the animal if possible and immediately collect the blood from the aorta or vena cava. Collecting blood from a severed head is never acceptable.  Blood obtained from bleeding the tail is also generally unacceptable.  Tissue fluids and other extraneous debris will often result in an activation of the coagulation cascase.  Blood drawn into a vacuum tube is the best source of blood.  See the product insert for Histopaque® 1083 for the amount of anticoagulant to add to blood collected with a syringe.

  7. Can Product 10831, Histopaque®-1083, be used with human blood?

    Cells can be recovered from human blood using Histopaque® 1083.  However Sigma sells Histopaque® 1077 specifically for use with human blood.  Histopaque® 1077 is intended for In Vitro Diagnostic Use.  Histopaque® 1083 is intended for research use only with animal blood.

  8. Is there any data for expected percent recovery with rat or mouse blood when using Product 10831, Histopaque®-1083?

    No.  Histopaque® 1083 is a research use product and no studies have been performed to determine percent recovery.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Gangduo Wang et al.
Toxicology and applied pharmacology, 237(2), 188-195 (2009-04-01)
Even though reactive oxygen and nitrogen species (RONS) are implicated as mediators of autoimmune diseases (ADs), little is known about contribution of protein oxidation (carbonylation and nitration) in the pathogenesis of such diseases. The focus of this study was, therefore
Bella S Guerrouahen et al.
Pediatric blood & cancer, 53(6), 1132-1135 (2009-06-02)
Tyrosine kinase inhibitors, such as imatinib, have dramatically improved the outcomes for patients with selected cancers. For imatinib, western blotting of phospho-CrkL was an insensitive, indirect, and descriptive method to determine drug efficacy. Greater use of targeted therapies should involve
Ahmad J H Albaghdadi et al.
Scientific reports, 9(1), 6528-6528 (2019-04-27)
Polycystic ovary syndrome (PCOS) is characterized by failure of ovulation and is associated with obesity and chronic inflammation. Recent evidence suggests that anomalous activation of ovarian macrophages and numerical and functional deficits in the Th17 (CD4+IL17A+) and the CD4+CD25+CD127low Tregs
Qiyue Hu et al.
Scientific reports, 8(1), 7675-7675 (2018-05-18)
Interleukin-15 (IL-15) can promote both innate and adaptive immune reactions by stimulating CD8+/CD4+ T cells and natural killer cells (NK) while showing no effect in activating T-regulatory (Treg) cells or inducing activation-associated death among effector T cells and NK cells.
Ulrich Flögel et al.
Circulation, 118(2), 140-148 (2008-06-25)
In this study, we developed and validated a new approach for in vivo visualization of inflammatory processes by magnetic resonance imaging using biochemically inert nanoemulsions of perfluorocarbons (PFCs). Local inflammation was provoked in 2 separate murine models of acute cardiac
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实验方案

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Histopaque® 密度梯度介质故障排除指南

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。

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