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17783

Sigma-Aldrich

钙黄绿素-AM

BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

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别名:
钙黄绿素乙酰氧基甲酯, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester
经验公式（希尔记法）:
C46H46N2O23
CAS号:
148504-34-1
分子量:
994.86
MDL编号:
MFCD05861516
PubChem化学物质编号:
57647670
NACRES:
NA.32

产品线

BioReagent

检测方案

≥95.0% (HPLC)

荧光

λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+

适用性

suitable for fluorescence

储存温度

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3

InChI key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

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相关类别

一般描述

脂质体含有一种自淬灭荧光染料，称为钙黄绿素。它是一种荧光染料，激发和发射波长分别为495/515 nm。它在浓度高于70mM时发生自淬灭，通常用作脂质囊泡渗漏的指示剂。当捕获的钙黄绿素从脂质体区室泄漏时，可根据荧光增加了计算泄漏速率。它被用作络合物指示剂，用来滴定EDTA螯合的钙离子，并且用于通过荧光检测法测定钙离子浓度。

应用

钙黄绿素-AM已被用于流式细胞术。
钙黄绿素的非荧光细胞渗透衍生物，水解后发出荧光。 用作多药耐药蛋白MRP的中性底物；用于肿瘤研究。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Calcein-AM, Product 17783?

    Sigma tests the solubility of Product No. 17783 at a concentration of 10 mg/mL in dimethyl sulfoxide (DMSO).

  6.  What is the appearance of Calcein-AM, Product No. 17783?

    Product No. 17783 can be a film, oil, paste or solid.

  7. What are the spectral properties of Calcein-AM, Product 17783?

    The emission maximum for Calcein-AM is 516 nm, with excitation at 496 nm in 0.1M Tris pH 8.0 (following hydrolysis by esterase enzyme, bound to Ca(2+)).

  8. How is Calcein-AM, Product 17783, typically used?

    Calcein-AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein-AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.

  9. Do the Scienceware® brand polypropylene products contain any leachable material?  

    Although Bel-Art does not specifically test their Scienceware® brand polypropylene products, they have no evidence of any problems with leachable material in the polypropylene.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Y S Tsao et al.
Biochemistry, 24(5), 1092-1098 (1985-02-26)
Sendai virus induced liposome leakage has been studied by using liposomes containing a self-quenching fluorescent dye, calcein. The liposomes used in this study were prepared by a freeze and thaw method and were composed of phosphatidylcholine, phosphatidylserine, and phosphatidylethanolamine (1:2.60:1.48
T M Allen et al.
Biochimica et biophysica acta, 597(2), 418-426 (1980-04-10)
Efflux of contents from small unilamellar vesicles of various compositions, conaining a highly quenched fluorescent compound (calcein, 175 mM) was determined as a function of temperature in the presence and absence of human serum. Efflux of calcein from the liposomes
Xiao-Cong Huang et al.
Biochemical pharmacology, 85(9), 1257-1268 (2013-02-19)
High intrinsic or acquired expression of membrane spanning, adenosine triphosphate binding cassette (ABC) transporter proteins, such as P-glycoprotein (P-gp), in cancers represents a major impediment to chemotherapy, with accelerated drug efflux leading to multi-drug resistance (MDR). Although ABC transporter inhibitors
Bao-Ling Du et al.
Journal of biomedical materials research. Part A, 103(4), 1533-1545 (2014-07-22)
Biological materials combined with genetically-modified neural stem cells (NSCs) are candidate therapy targeting spinal cord injury (SCI). Based on our previous studies, here we performed gelatin sponge (GS) scaffold seeded with neurotrophin-3 (NT-3) and its receptor TrkC gene modifying NSCs
Johan van der Stok et al.
Tissue engineering. Part A, 21(9-10), 1495-1506 (2015-01-30)
A promising bone graft substitute is porous titanium. Porous titanium, produced by selective laser melting (SLM), can be made as a completely open porous and load-bearing scaffold that facilitates bone regeneration through osteoconduction. In this study, the bone regenerative capacity
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