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Merck
CN

32670

Sigma-Aldrich

4′,6-二脒基-2-苯基吲哚 二盐酸盐

BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

别名:

2-(4-氨基苯基)-6-吲哚甲酰胺 二盐酸盐, DAPI 二盐酸盐

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About This Item

经验公式(希尔记法):
C16H15N5 · 2HCl
CAS号:
分子量:
350.25
Beilstein:
4894417
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.32

产品线

BioReagent

质量水平

检测方案

≥95.0% (HPLC)

形式

powder

溶解性

DMF: soluble
H2O: soluble

荧光

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

适用性

suitable for fluorescence

储存温度

room temp

SMILES字符串

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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应用

在琼脂糖凝胶中染色DNA,DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱,通过对双链复合体的特殊可视化检测印迹应用中的退火探针,并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。

生化/生理作用

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟(富含AT的DNA优先)结合,形成稳定的复合物,其发出的荧光比单独的DAPI高约20倍。

包装

无底玻璃瓶。内含物在插入的融合锥体内。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can I make a solution of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?

    DAPI is soluble in water (10 mg/mL) with heat or sonication as necessary. It is insoluble in PBS even at 1 mg/ml.

  4. How can I store solutions of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?

    At 1 mg/mL, the solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored frozen at -20°C

  5. What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear counterstain for tissue sections?

    Following fluorescent staining of the cells, DAPI is added to the mounting media at 2 μg/ml.

  6. What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear stain for cultured cells?

    Use DAPI at 0.1 microgram per mL in phosphate buffered saline (PBS) to incubate with monolayer cultured cells for 15-30 minutes at 30°C.  Immediately examine with a fluorescence microscope.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Science advances, 5(5), eaav1594-eaav1594 (2019-05-21)
Polycomb repressive complexes are evolutionarily conserved complexes that maintain transcriptional repression during development and differentiation to establish and preserve cell identity. We recently described the fundamental role of PRC1 in preserving intestinal stem cell identity through the inhibition of non-lineage-specific
Minggang Fang et al.
Journal of virology, 83(23), 12569-12578 (2009-09-18)
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac96 is a core gene, but its role in virus replication is still unknown. To determine its role in the baculovirus life cycle, we used the AcMNPV bacmid system to generate an ac96-null virus (vAc(96)(null)).
Angus T Stock et al.
The Journal of experimental medicine, 211(5), 751-759 (2014-04-23)
Infection induces the expression of inflammatory chemokines that recruit immune cells to the site of inflammation. Whereas tissues such as the intestine and skin express unique chemokines during homeostasis, whether different tissues express distinct chemokine profiles during inflammation remains unclear.
Sanna Koskiniemi et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(17), 7032-7037 (2013-04-11)
Rearrangement hotspot (Rhs) and related YD-peptide repeat proteins are widely distributed in bacteria and eukaryotes, but their functions are poorly understood. Here, we show that Gram-negative Rhs proteins and the distantly related wall-associated protein A (WapA) from Gram-positive bacteria mediate
François Binet et al.
Cell metabolism, 17(3), 353-371 (2013-03-12)
In stroke and proliferative retinopathy, despite hypoxia driven angiogenesis, delayed revascularization of ischemic tissue aggravates the loss of neuronal function. What hinders vascular regrowth in the ischemic central nervous system remains largely unknown. Using the ischemic retina as a model

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