跳转至内容
Merck
CN

32670

4′,6-二脒基-2-苯基吲哚 二盐酸盐

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

别名:

2-（4-氨基苯基）-6-吲哚甲酰胺 二盐酸盐, DAPI 二盐酸盐

登录 查看组织和合同定价。

选择尺寸

关于此项目

经验公式（希尔记法）:
C16H15N5 · 2HCl
化学文摘社编号:
28718-90-3
分子量:
350.25
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
57648699
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
MFCD00012681

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

4′,6-二脒基-2-苯基吲哚 二盐酸盐, suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

product line

BioReagent

assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble
H2O: soluble

fluorescence

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

suitability

suitable for fluorescence

storage temp.

room temp

Quality Level

100

正在寻找类似产品? 访问 产品对比指南

相关类别

Application

在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。
在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱，通过对双链复合体的特殊可视化检测印迹应用中的退火探针，并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。

Biochem/physiol Actions

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟（富含AT的DNA优先）结合，形成稳定的复合物，其发出的荧光比单独的DAPI高约20倍。

Packaging

无底玻璃瓶。内含物在插入的融合锥体内。

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCF5587
BCBZ8675
BCBS7582V
BCBP8312V
BCBL9207V

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can I make a solution of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?

    DAPI is soluble in water (10 mg/mL) with heat or sonication as necessary. It is insoluble in PBS even at 1 mg/ml.

  4. How can I store solutions of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?

    At 1 mg/mL, the solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored frozen at -20°C

  5. What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear counterstain for tissue sections?

    Following fluorescent staining of the cells, DAPI is added to the mounting media at 2 μg/ml.

  6. What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear stain for cultured cells?

    Use DAPI at 0.1 microgram per mL in phosphate buffered saline (PBS) to incubate with monolayer cultured cells for 15-30 minutes at 30°C.  Immediately examine with a fluorescence microscope.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Silvia Pivetti et al.
Science advances, 5(5), eaav1594-eaav1594 (2019-05-21)
Polycomb repressive complexes are evolutionarily conserved complexes that maintain transcriptional repression during development and differentiation to establish and preserve cell identity. We recently described the fundamental role of PRC1 in preserving intestinal stem cell identity through the inhibition of non-lineage-specific
Minggang Fang et al.
Journal of virology, 83(23), 12569-12578 (2009-09-18)
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac96 is a core gene, but its role in virus replication is still unknown. To determine its role in the baculovirus life cycle, we used the AcMNPV bacmid system to generate an ac96-null virus (vAc(96)(null)).
Georgia Pennarossa et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(22), 8948-8953 (2013-05-23)
The differentiated state of mature cells of adult organisms is achieved and maintained through the epigenetic regulation of gene expression, which consists of several mechanisms including DNA methylation. The advent of induced pluripotent stem cell technology enabled the conversion of
Shriparna Sarbajna et al.
Genes & development, 28(10), 1124-1136 (2014-05-17)
The resolution of recombination intermediates containing Holliday junctions (HJs) is critical for genome maintenance and proper chromosome segregation. Three pathways for HJ processing exist in human cells and involve the following enzymes/complexes: BLM-TopoIIIα-RMI1-RMI2 (BTR complex), SLX1-SLX4-MUS81-EME1 (SLX-MUS complex), and GEN1.
Xiaohong Zhang et al.
Development (Cambridge, England), 141(17), 3352-3362 (2014-08-01)
FGF signaling is essential for mammary gland development, yet the mechanisms by which different members of the FGF family control stem cell function and epithelial morphogenesis in this tissue are not well understood. Here, we have examined the requirement of
查看所有相关文献

商品

Detect, Visualize and Quantify Single Post-Translational Modifications

Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications

相关内容

DNA Detection

Nancy-520 for DNA Detection and Quantitation

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持