Merck
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35845

Sigma-Aldrich

2′,7′-二氯荧光素二乙酸酯

BioReagent, suitable for fluorescence, ≥95% (HPLC)

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别名:
DCFHDA, 双氯荧光黄乙酸乙酯
经验公式（希尔记法）:
C24H14Cl2O7
CAS号:
2044-85-1
分子量:
485.27
Beilstein:
68488
MDL编号:
MFCD00037501
PubChem化学物质编号:
57649438
NACRES:
NA.32

产品线

BioReagent

质量水平

100

检测方案

≥95% (HPLC)

mp

232-234 °C (lit.)

溶解性

DMSO: soluble

荧光

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

InChI key

VQVUBYASAICPFU-UHFFFAOYSA-N

相关类别

一般描述

2′,7′-二氯荧光素二乙酸酯，也称为 DCFHDA 或 Dcfh-DA，是一种敏感的细胞可渗透氧化还原探针。亲脂性非荧光 Dcfh-DA 通过被动扩散和脱乙酰作用很容易穿过细胞膜。脱酰产物是一种对氧化剂敏感的 2′,7′-二氯荧光素（DCHF）。 DCHF 随后被氧化以形成高荧光 DCF，这是被测量的。

应用

2′,7′-二氯荧光素二乙酸酯主要用于检测活性氧（ROS）中间体并量化细胞中的过度 氧化应激 。

Dcfh-DA 用于定量测定，以测量纳米颗粒处理的细胞中的氧化应激。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?

    2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.

  4. In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?

    It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).

  5. What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?

    Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).

  6. What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?

    Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?

    Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis. 

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    Ask a Scientist here.

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