主要文件

查看所有文档

35848

2′,7′-二氯荧光素

Name: 2′,7′-二氯荧光素
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (T)

别名:

DCF, 二氯荧光黄

登录查看组织和合同定价。

选择尺寸

变更视图

关于此项目

经验公式（希尔记法）:

C₂₀H₁₀Cl₂O₅

化学文摘社编号:

76-54-0

分子量:

401.20

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57649439

EC Number:

200-968-6

Beilstein/REAXYS Number:

58009

MDL number:

MFCD00005047

技术服务

需要帮助？我们经验丰富的科学家团队随时乐意为您服务。

让我们为您提供帮助

product line

BioReagent

Quality Level

100

assay

≥90% (T)

form

solid

mp

280 °C (dec.) (lit.)

fluorescence

λ_ex 504 nm; λ_em 529 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

正在寻找类似产品? 访问产品对比指南

General description

2′,7′-二氯荧光素，也称为 DCF，是一种荧光染料。DCF 荧光激发波长为 498nm，发射波长为 522nm。亲脂性非荧光 Dcfh-DA 通过被动扩散和脱乙酰作用很容易穿过细胞膜。脱酰产物是一种对氧化剂敏感的 2′,7′-二氯荧光素（DCHF）。 DCHF 随后被氧化以形成高荧光 DCF，这是被测量的。

Application

2′,7′-二氯荧光素用作荧光探针，用于测量 ROS（活性氧），用于生物学研究。它也可作为预浸剂，用于荧光测定邻位二醇、糖苷和酚的预浸剂。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

H. Watanabe et al.

Analytical Sciences, 2, 461-461 (1986)

W.D. Pfeffer et al.

Journal of Chromatography A, 506, 401-401 (1990)

Fluorescence densitometric method for the determination of gluconic and lactobionic acids ("sugar acids") in pharmaceutical preparations.

G Gübitz et al.

Journal of chromatography, 117(2), 337-343 (1976-02-18)

An in situ fluorimetric method has been developed for the quantitation of gluconic and lactobionic acids and their salts in tablet formulations. The method is based on glycol cleavage with lead tetraacetate followed by treatment with dichlorofluorescein. Calcium gluconate and

Evidence for free radical formation during the oxidation of 2'-7'-dichlorofluorescin to the fluorescent dye 2'-7'-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements.

C Rota et al.

Free radical biology & medicine, 27(7-8), 873-881 (1999-10-09)

The oxidation of 2'-7'-dichlorofluorescin (DCFH) to the fluorescent 2'-7'-dichlorofluorescein (DCF) by horseradish peroxidase (HRP) was investigated by fluorescence, absorption, and electron spin resonance spectroscopy (ESR). As has been previously reported, HRP/H2O2 oxidized DCFH to the highly fluorescent DCF. However, DCF

2',7'-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy.

Xiuping Chen et al.

Free radical research, 44(6), 587-604 (2010-04-08)

Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However

查看所有相关文献

全球贸易项目编号

货号	GTIN
35848-5G	04061831812848
35848-25G	04061831812831

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持