Merck
CN

45359

Sigma-Aldrich

环氧包埋介质试剂盒

embedding resin for electron microscopy

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别名:
Epon 替代包埋介质试剂盒
NACRES:
NA.47

pH值(酸碱度)

5-7

密度

1.9 g/cm3

application(s)

hematology
histology

储存温度

2-8°C

一般描述

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

应用

Epoxy Embedding Medium Kit has been used in the following applications:

  • to study the role of NDRG2, an essential colonic epithelial barrier regulator, in gut homeostasis maintenance and colitis-associated tumor development
  • to study the interaction of polymer scaffold nanofibers with growing cells
  • to study the morphological characterization of postembryonic development of blood–spleen barrier in ducks
  • to understand the role of NOD1/NOD2 receptors in Fusobacterium nucleatum-mediated neutrophil extracellular traps

特点和优势

  • Excellent medium for embedding a wide variety of tissues, including both plant and animal.
  • Facilitates rapid embedding (less than 3 hours).
  • Low viscosity facilitates rapid tissue penetration.
  • Desirable hardness is easy to obtain to suit a specific tissue type.

组分

45345 环氧包埋剂 2 x 250 mL
45347 硬化剂 MNA 250 mL
45346 硬化剂 DDSA 250 mL
45348 加速器 DMP 30 250 mL

法律信息

Epon is a trademark of Hexion, Inc.

相关产品

产品编号
说明
价格

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 4 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

275.0 °F

闪点(°C)

135 °C


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1000309185

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Konstantin E Mochalov et al.
Ultramicroscopy, 182, 118-123 (2017-07-04)
In the past decade correlative microscopy, which combines the potentials of different types of high-resolution microscopies with a variety of optical microspectroscopy techniques, has been attracting increasing attention in material science and biological research. One of outstanding solutions in this
Kunio Nagashima et al.
Methods in molecular biology (Clifton, N.J.), 697, 83-91 (2010-12-01)
This chapter outlines the procedures for ex vivo TEM preparation of nanoparticle-containing tissue or cell culture samples using an epoxy resin embedding method. The purpose of this procedure is to preserve the structure of tissue in a hardened epoxy block
Arnold Lavaud et al.
Translational vision science & technology, 9(5), 13-13 (2020-08-22)
To evaluate visual streak (VS) identification on spectral-domain optical coherence tomography (SD-OCT) scans in awake rabbits. To report thickness measurements in the VS and adjacent retina on OCT B-scans and histologic sections and to assess inter-method bias, precision and repeatability
A Sawaguchi et al.
Journal of microscopy, 234(2), 113-117 (2009-04-29)
The goal of specimen preparation for transmission electron microscopy is to obtain high-quality ultra-thin sections with which we can correlate cellular structure to physiological function. In this study, we newly developed a capsule-supporting ring that can be useful for resin
Sevinç Kenan et al.
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 47(3), 473-483 (2019-01-09)
This study investigates the effects of semaphorin 3A on new bone formation in an experimental rat model. Cortical bone defects, 5 mm, were created in the calvaria of 40 Wistar rats, which were then separated into three groups: empty defect (control)

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