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Merck
CN

46940

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯

suitable for fluorescence, ≥75% (HPLC)

别名:

荧光素-5 (6)-氨基己酸 N-琥珀酰亚胺酯

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关于此项目

经验公式（希尔记法）:
C31H26N2O10
化学文摘社编号:
114616-31-8
分子量:
586.55
NACRES:
NA.32
PubChem Substance ID:
329757270
UNSPSC Code:
12352108
MDL number:
MFCD00467542

主要文件

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产品名称

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯, suitable for fluorescence, ≥75% (HPLC)

InChI

1S/2C31H26N2O10/c34-18-6-9-22-24(15-18)41-25-16-19(35)7-10-23(25)31(22)21-8-5-17(14-20(21)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37;34-18-6-9-21-24(15-18)41-25-16-19(35)7-10-22(25)31(21)23-14-17(5-8-20(23)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h2*5-10,14-16,34-35H,1-4,11-13H2,(H,32,39)

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)C(=O)NCCCCCC(=O)ON6C(=O)CCC6=O)c1.Oc7ccc8c(Oc9cc(O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)NCCCCCC(=O)ON%12C(=O)CCC%12=O)c7

InChI key

WAGYELYAQOYZFU-UHFFFAOYSA-N

assay

≥75% (HPLC)

form

solid

solubility

DMF: soluble
methanol: soluble

fluorescence

λex 490 nm; λem 514 nm (pH 8.2)
λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

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相关类别

Application

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯（FHS）作为试剂将荧光团6-[荧光素-5(6)-甲酰胺基]己酸偶联到分子上。6-[荧光素-5 (6)-甲酰胺基] 己酸用作基于荧光素的蛋白质荧光标记物，可在猝灭实验等应用中进行分析。
引入荧光素标记的试剂；间隔基使反应性基团更易于生物聚合物上的亲核试剂接触

General description

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯（FNHS）是一种荧光标签，又称荧光素。FNHS 最大激发波长为 490 nm，最大发射波长为 514 nm。 6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯与伯胺共价结合。

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCH5965
BCCK7257
BCCD3685
BCBX6012
BCBS2220V

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R Bertrand et al.
Biochemistry, 34(29), 9500-9507 (1995-07-25)
We describe, for the first time, the reaction of skeletal myosin subfragment 1 (S-1) with the succinimido ester of 6-[fluorescein-5(and 6)-carboxamido]hexanoic acid (FHS), which takes place at pH 7.0, 20 degrees C, within a 15 min period, in the presence
Theresa R Bomfim et al.
Archives of biochemistry and biophysics, 505(1), 105-111 (2010-10-05)
2,4-Dinitrophenol (DNP) increases the affinity of myosin for actin and accelerates its Mg(2+)ATPase activity, suggesting that it acts on a region of the myosin head that transmits conformational changes to actin- and ATP-binding sites. The binding site/s for DNP are
A M Vaz et al.
Colloids and surfaces. B, Biointerfaces, 114, 11-19 (2013-10-29)
In this work, we present a suitable methodology to produce magnetically recoverable bioreactors based on enzymes, which are covalently attached on the surface of iron oxide@silica nanoparticles. In order to produce this system, iron oxide clusters with a mean diameter
Avidin binding of carboxyl-substituted biotin and analogues.
K Hofmann et al.
Biochemistry, 21(5), 978-984 (1982-03-02)
A G Johansson et al.
Hepatology (Baltimore, Md.), 24(1), 169-175 (1996-07-01)
Immune complexes were formed between dinitrophenylated human serum albumin (DNP-HSA) and polyclonal rabbit immunoglobulin G (IgG) anti-DNP antibodies at antibody excess. The antigen was labelled with isotope (125I-tyramine-cellobiose) or fluorochrome, (6-[fluorescein-5-(and-6)-carboxamido] hexanoic-acid, succinimidyl ester). The radiolabelled antigen, native or antibody
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