Merck
CN

46955

Sigma-Aldrich

荧光素

for fluorescence, free acid

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别名:
酸性黄 73
经验公式(希尔记法):
C20H12O5
CAS号:
分子量:
332.31
颜色索引号:
45350
Beilstein:
94324
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.32

等级

for fluorescence

质量水平

technique(s)

titration: suitable

荧光

λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0

适用性

suitable for fluorescence

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChI key

GNBHRKFJIUUOQI-UHFFFAOYSA-N

Gene Information

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一般描述

荧光素染料是最常用的荧光探针。它可以为上皮缺陷染色,分析角膜上皮的完整性。荧光素用于角膜穿孔的诊断。口服或静脉注射荧光素在荧光素血管造影术中用于检测糖尿病视网膜病变和脉络膜新生血管膜。

应用

荧光素已用于:
  • Caco-2电池的渗透性检测
  • 用于测试终生荧光标准品的适用性
  • 作为荧光团,用于在存在血红蛋白时迅速恢复荧光峰值和荧光浓度
  • 在氧自由基吸收能力(ORAC)检测中作为荧光探针

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

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1000309185

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  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Product 46955, Fluorescein, soluble in?

    According to the chemicals encyclopedia published by the Royal Society of Chemistry : 13th Edition, Fluorescein is soluble in hot alcohol and glacial acetic acid. Also, it is soluble in alkali hydroxides or carbonates. This product is insoluble in water, benzene, chloroform and ether.

  4. How should Product 46955, Fluorescein, be stored?

    We recommend storing this product at room temperature.

  5. What is the dye content of Product 46955, Fluorescein?

    According to our supplier, the dye content of pure Fluorescein is a minimum of 90%. We do not test the purity of this product since it is not one of our specifications.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Fluorescein, Quinine Sulfate and Green Fluorescent Protein
Kristoffersen AS, et al.
Journal of Fluorescence, 28(5), 1065-1073 (2018)
Hirofumi Shoda et al.
Arthritis & rheumatology (Hoboken, N.J.), 67(5), 1171-1181 (2015-03-18)
The balance between effector and regulatory CD4+ T cells plays a key role in the pathogenesis of rheumatoid arthritis (RA). The aim of this study was to examine whether the RA autoantigen BiP has epitopes for both effector and regulatory
Experimental recovery of intrinsic fluorescence and fluorophore concentration in the presence of hemoglobin: spectral effect of scattering and absorption on fluorescence
Du Le VN, et al.
Journal of Biomedical Optics, 20(12), 127003-127003 (2015)
Diagnostic agents
Robert-Gnansia E
Drugs During Pregnancy and Lactation, 502-513 (2007)
Microfluidic gut-on-a-chip with three-dimensional villi structure
Shim KY, et al.
Biomedical Microdevices, 19(2), 37-37 (2017)

商品

荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析,感测和FLI。

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Developed in the last several years, fluorescence quenching microscopy (FQM) has enabled rapid, inexpensive, and high-fidelity visualization of two-dimensional (2D) materials such as graphene-based sheets and MoS2.

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