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Merck
CN

51411C

SAFC

最低必需培养基

with Earle′s Balanced Salts, with 2.0 mM L-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

Eagle最低必需培养基, MEM

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UNSPSC代码:
12352207
NACRES:
NA.75
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描述

for research or for further manufacturing use

质量水平

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

组分

Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
NaHCO3: 2200 mg/L
L-glutamine: 290 mg/L

运输

ambient

储存温度

2-8°C

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一般描述

Harry Eagle研究了小鼠L细胞和HeLa细胞在培养中的基本营养需求,因此开发了含有Earle′s平衡盐的最低必需培养基(MEM)。 他证明这些细胞需要13种氨基酸和7种维生素才能在体外生长和繁殖。 MEM是Eagle′s早期培养基基础培养基Eagle(BME)的改良,含有更高浓度的必需营养素。 这些培养基已证明能够在培养中支持多种正常细胞和转化细胞,并含有Earle′s平衡盐,这使其适合在装有CO2的大气中使用。

储存分类代码

12 - Non Combustible Liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基
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