产品名称
杜氏改良培养基-高葡萄糖, with 4500 mg/L dextrose, with 4.0 mM L-glutamine, with 110 mg/L sodium pyruvate, without sodium bicarbonate, dry powder, suitable for cell culture DME, DMEM, Dulbecco’s Modified Eagle’s Medium – high Glucose. Use 13.49 g/L See information sheet, 56499C @ www.sigmaaldrich.com for hydration instructions.
description
for research or for further manufacturing use
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。
Preparation Note
按照每升培养基13.5克粉末配制。
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存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable