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646563

DL-二硫代苏糖醇 溶液

1 M in H2O

别名:

(±)-苏式-1,4-二巯基-2,3-丁二醇 溶液, DTT

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关于此项目

经验公式（希尔记法）:
C4H10O2S2
化学文摘社编号:
3483-12-3
分子量:
154.25
UNSPSC Code:
12161504
NACRES:
NA.25
PubChem Substance ID:
24883472
MDL number:
MFCD00004877
Beilstein/REAXYS Number:
1719757
Form:
liquid
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form

liquid

Quality Level

100

reaction suitability

reagent type: reductant

concentration

1 M in H2O

density

1.04 g/mL at 20 °C, 1.035 g/mL at 25 °C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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General description

二硫苏糖醇是用于蛋白质分析的一种有效还原剂。在制备用于电泳或其他应用的样品时，密封安瓿形式更加方便且易于使用，并且可避免浪费和效力损失。
二硫苏糖醇（DTT）在蛋白质组学应用中用于保护还原态中的巯基（-SH），也用于二硫基（-S-S-）基团的定量还原。由于其能够还原蛋白质样品的氧化态并保留酶活性，它是一种常用的缓冲液试剂。在溶液中其它二硫化物的还原过程中，DTT被氧化为环状二硫化物。

Application

DL-二硫苏糖醇溶液已被用于：
  • 蛋白质印迹
  • 作为裂解缓冲液的增补剂用于研究其对精子裂解的作用
  • 作为冷冻均质缓冲液和冷冻冲洗缓冲液的成分用于分离冷冻大鼠组织的细胞核
  • 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、色谱、含半胱氨酸化合物的修饰

Features and Benefits

与其它硫醇化合物相比具有低毒性和减少的异味。

pictograms

Corrosion
GHS05

signalword

Danger

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

存储类别

8B - Non-combustible corrosive hazardous materials

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKDB0645
MKCZ4629
MKCX8562
MKCX7749
MKCX3447

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货号GTIN
646563-10X.5ML04061832730349

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