Merck
CN

646563

Supelco

DL-二硫代苏糖醇 溶液

1 M in H2O

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别名:
(±)-苏式-1,4-二巯基-2,3-丁二醇 溶液, DTT
Empirical Formula (Hill Notation):
C4H10O2S2
CAS号:
分子量:
154.25
Beilstein:
1719757
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

形式

liquid

质量水平

reaction suitability

reagent type: reductant

浓度

1 M in H2O

密度

1.04 g/mL at 20 °C
1.035 g/mL at 25 °C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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此商品
D977943816D0632
DL-Dithiothreitol solution 1 M in H2O

Supelco

646563

DL-二硫代苏糖醇 溶液

-
DL-Dithiothreitol for molecular biology, ≥98% (HPLC), ≥99% (titration)

Sigma-Aldrich

D9779

DL-二硫苏糖醇

-
DL-Dithiothreitol solution BioUltra, for molecular biology, ~1 M in H2O

Sigma-Aldrich

43816

DL-二硫代苏糖醇 溶液

Premium Grade
DL-Dithiothreitol ≥98% (HPLC), ≥99.0% (titration)

Sigma-Aldrich

D0632

DL-二硫苏糖醇

-
reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

density

1.04 g/mL at 20 °C, 1.035 g/mL at 25 °C

density

-

density

1.04 g/mL at 20 °C

density

-

concentration

1 M in H2O

concentration

-

concentration

~1 M in H2O

concentration

-

一般描述

二硫苏糖醇是用于蛋白质分析的一种有效还原剂。在制备用于电泳或其他应用的样品时,密封安瓿形式更加方便且易于使用,并且可避免浪费和效力损失。
二硫苏糖醇(DTT)在蛋白质组学应用中用于保护还原态中的巯基(-SH),也用于二硫基(-S-S-)基团的定量还原。由于其能够还原蛋白质样品的氧化态并保留酶活性,它是一种常用的缓冲液试剂。在溶液中其它二硫化物的还原过程中,DTT被氧化为环状二硫化物。

应用

DL-二硫苏糖醇溶液已被用于:
  • 蛋白质印迹
  • 作为裂解缓冲液的增补剂用于研究其对精子裂解的作用
  • 作为冷冻均质缓冲液和冷冻冲洗缓冲液的成分用于分离冷冻大鼠组织的细胞核
  • 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、色谱、含半胱氨酸化合物的修饰

特点和优势

与其它硫醇化合物相比具有低毒性和减少的异味。

配件

产品编号
说明
价格

象形图

Corrosion

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Skin Irrit. 2

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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L-(−)-Dithiothreitol ≥95% (titration)

Sigma-Aldrich

D9760

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Sigma-Aldrich

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碘乙酰胺

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Millipore

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Sigma-Aldrich

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