67060
甲基绿
zinc chloride salt, suitable for microscopy
别名:
乙基绿
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线性分子式:
C27H35BrClN3 · ZnCl2
化学文摘社编号:
分子量:
653.24
EC Number:
230-415-4
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
42590
Beilstein/REAXYS Number:
3914106
InChI key
RRKSQWOQZFIQKW-UHFFFAOYSA-J
InChI
1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
SMILES string
[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
form
powder
technique(s)
titration: suitable
pH
0.2-1.8, yellow to blue
mp
>300 °C (lit.)
solubility
water: 0.01 g/10 mL, blue to very deep blue
εmax
≥900 at 624-634 nm in water
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Application
Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.
Biochem/physiol Actions
Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.
Analysis Note
Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
target_organs
Respiratory system
存储类别
8A - Combustible corrosive hazardous materials
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
Monocyte chemoattractant protein-1 in human atheromatous plaques.
Nelken NA, et al.
The Journal of Clinical Investigation, 88, 1121-1121 (1991)
Evaluation of apoptosis as a mechanism of follicular cell atresia in the ovaries of cattle (bos indicus) and buffalo (bubalus bubalis) fetuses.
Santos SSD, et al.
Animal Reproduction, 10, 55-61 (2013)
K R Fox et al.
European journal of histochemistry : EJH, 36(3), 263-270 (1992-01-01)
Methyl green has long been used as a DNA stain in histochemistry. The sequence selective binding of the cationic triphenylmethane dyes methyl green, crystal violet and Malachite green to DNA was investigated by DNAase 1 and micrococcal nuclease footprinting. At
Chun-Chi Liang et al.
The Journal of clinical investigation, 124(7), 3080-3092 (2014-06-18)
Lack of a preclinical model of primary dystonia that exhibits dystonic-like twisting movements has stymied identification of the cellular and molecular underpinnings of the disease. The classical familial form of primary dystonia is caused by the DYT1 (ΔE) mutation in
Nasim Shweke et al.
The American journal of pathology, 173(3), 631-642 (2008-08-09)
Renal fibrosis is defined by the exaggerated accumulation of extracellular matrix proteins. Tissue transglutaminase (TG2) modifies the stability of extracellular matrix proteins and renders the extracellular matrix resistant to degradation. In addition, TG2 also activates transforming growth factor-beta (TGF-beta). We
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