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Merck
CN

75259

Sigma-Aldrich

三 2-羰基乙基 磷盐酸盐 盐酸盐

BioUltra, ≥98% (NMR)

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别名:
TCEP
经验公式(希尔记法):
C9H15O6P · HCl
CAS号:
分子量:
286.65
Beilstein:
3724376
MDL编号:
UNSPSC代码:
12352128
PubChem化学物质编号:
NACRES:
NA.25

产品线

BioUltra

质量水平

检测方案

≥98% (NMR)

形式

crystals

反应适用性

reagent type: reductant

缺失

≤1.0% loss on drying

pH值(酸碱度)

1.2-1.5

溶解性

H2O: 1 M, clear, colorless (no residue)

痕量阴离子

sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤5 mg/kg
As: ≤0.05 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in H2O

紫外吸收

λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03

储存温度

2-8°C

SMILES字符串

Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

InChI key

PBVAJRFEEOIAGW-UHFFFAOYSA-N

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一般描述

三(2-羧乙基)磷 盐酸盐(TCEP)是一种强还原剂,可在水溶液中稳定。它很容易以高特异性的方式按化学计量还原二硫化物。TCEP在pH 1.5到pH 9.0时表现出还原性,并在pH 4时还原脱氧抗坏血酸(DHA)。

应用

三(2-羧乙基)磷 盐酸盐被用于:
  • 作为IRE1透析缓冲液、RNA裂解缓冲液的组分用于 体外 转录RNA的 体外 裂解试验
  • 在还原巯基得到总硫醇部分
  • 还原蛋白质样品

水溶性试剂,用于二硫化物的选择性还原。在质谱应用中比 DTT 更稳定。

象形图

Corrosion

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Skin Corr. 1B

WGK

WGK 1


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