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Merck
CN

77151

胃蛋白酶 来源于猪胃粘膜

powder, slightly beige, 1200-2400 U/mg

别名:

胃液素 来源于猪胃, 胃蛋白酶 A

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010127
EC Number:
232-629-3
NACRES:
NA.54
MDL number:
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产品名称

胃蛋白酶 来源于猪胃粘膜, powder, slightly beige, 1200-2400 U/mg

form

powder

specific activity

1200-2400 U/mg

mol wt

35 kDa

color

slightly beige

UniProt accession no.

storage temp.

2-8°C

Gene Information

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Analysis Note

最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。

Application

胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

Biochem/physiol Actions

优先裂解:P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键

Disclaimer

可能适用相应的销售限制。

Other Notes

在pH 2.0和37℃条件下,1 U对应于每分钟使280 nm处的吸光度增加0.001的酶量(血红蛋白,货号51290,作为底物)如上所述的15′000吸光度-U相当于 ∼1 Bergmeyer-U。 1 Bergmeyer-U是在pH 2.0和37℃条件下水解1 μmmol乙酰基-L-苯基-3,5-二碘代-L-酪氨酸的酶量

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品
低风险生物材料
动植物源性产品
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