产品名称
胃蛋白酶 来源于猪胃粘膜, powder, slightly beige, 1200-2400 U/mg
form
powder
specific activity
1200-2400 U/mg
mol wt
35 kDa
color
slightly beige
UniProt accession no.
storage temp.
2-8°C
Gene Information
pig ... LOC396892(396892)
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Analysis Note
最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。
Application
胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer。
Biochem/physiol Actions
优先裂解:P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键
Disclaimer
可能适用相应的销售限制。
Other Notes
在pH 2.0和37℃条件下,1 U对应于每分钟使280 nm处的吸光度增加0.001的酶量(血红蛋白,货号51290,作为底物)如上所述的15′000吸光度-U相当于 ∼1 Bergmeyer-U。 1 Bergmeyer-U是在pH 2.0和37℃条件下水解1 μmmol乙酰基-L-苯基-3,5-二碘代-L-酪氨酸的酶量
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
低风险生物材料
动植物源性产品
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