77160
胃蛋白酶 来源于猪胃粘膜
powder, slightly beige, ≥500 U/mg
别名:
胃液素 来源于猪胃, 胃蛋白酶 A
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010127
EC Number:
232-629-3
NACRES:
NA.54
MDL number:
产品名称
胃蛋白酶 来源于猪胃粘膜, powder, slightly beige, ≥500 U/mg
form
powder
specific activity
≥500 U/mg
mol wt
35 kDa
color
slightly beige
UniProt accession no.
storage temp.
2-8°C
Gene Information
pig ... LOC396892(396892)
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Analysis Note
最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。
Application
胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。
Biochem/physiol Actions
优先裂解：P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键
Disclaimer
可能适用相应的销售限制。
Other Notes
1 个单位对应于在 pH 2.0 和 37℃ 下每分钟使 280 nm 处的吸光度增加 0.001 的酶量（血红蛋白，目录号 51290，作为底物）；上述 15,000 吸光度单位相当于约1 个 Bergmeyer 单位。一个Bergmeyer单位是在pH2.0和37℃下水解1 μmol乙酰基-L-苯基-3,5-二碘-L-酪氨酸的酶量。
多肽合成，综述。
肽合成，综述。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
低风险生物材料
常规特殊物品
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M.P. Bemquerer et al.
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Ludovic Hermabessiere et al.
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Plastic pollution is a source of chemical to the environment and wildlife. Despite the ubiquity of plastic pollution and thus plastic additive in the environment, plastic additives have been studied to a limited extend. As a prerequisite to a study
C A Abdel Malak et al.
International journal of peptide and protein research, 41(2), 97-101 (1993-02-01)
Swine pepsin at pH 5 efficiently catalyzes a condensation between Z-Ala-Ala-Phe-OH and p-nitroanilides of Leu, Phe, Val, Ala and Arg that leads to formation of corresponding benzyloxycarbonyl-tetrapeptide p-nitroanilides with yields of 70-90%. These reactions are complicated by co-precipitation of pepsin
J.S. Fruton
Carlsberg Research Communications, 49, 41-41 (1984)
Abdelhacib Kihal et al.
Animals : an open access journal from MDPI, 11(8) (2021-08-28)
The aim of this study was to determine the capacity of six mycotoxin binders (MTBs) to adsorb vitamins A, D and E in an in vitro system that simulates gastric and intestinal digestion. Experiment 1 evaluated the recovery rate of
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