产品名称
抗-小鼠 IgG - Atto 633 山羊抗, ~1 mg/mL IgG, affinity isolated antibody
biological source
goat
conjugate
Atto 633 conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
contains
50% glycerol as stabilizer
species reactivity
mouse
concentration
~1 mg/mL IgG
fluorescence
λex 636 nm; λem 650 nm in PBS
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Analysis Note
Unconjugated dye ≤5% of total fluorescence
Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spect
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Anti-mouse IgG (whole molecule) is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 633 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye. Atto 633-goat anti mouse IgG associates with mouse IgGs.
Anti-mouse IgG (whole molecule) is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 633 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye. Atto 633-goat anti mouse IgG associates with mouse IgGs.
Immunogen
purified mouse IgG (whole molecule).
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Physical form
Solution in phosphate buffered saline and 5 mM sodium azide, pH 7.5, with glycerol 1:1
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hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
Charlotte Calvet et al.
iScience, 25(12), 105628-105628 (2022-12-10)
Hearing depends on fast and sustained calcium-dependent synaptic vesicle fusion at the ribbon synapses of cochlear inner hair cells (IHCs). The implication of the canonical neuronal SNARE complex in this exocytotic process has so far remained controversial. We investigated the
Tharun Selvam Mahendran et al.
Frontiers in aging neuroscience, 12, 537712-537712 (2020-12-01)
Aggregated tau is a hallmark neuropathological feature in numerous neurodegenerative disorders. Previous studies aiming to validate aggregated tau pathology as a pathogenic driver of neurodegeneration in correlation to characteristic behavioral phenotypes have had shortcomings. Although studies on soluble tau pathology
Complement-fixing IgG1 constitutes a new subclass of mouse IgG.
P L Ey et al.
Nature, 281(5731), 492-493 (1979-10-11)
Structure and function of human and murine receptors for IgG.
J C Unkeless et al.
Annual review of immunology, 6, 251-281 (1988-01-01)
商品
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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