Ampliflu Red Westernblot Kit

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The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

Ampliflu is a trademark of Sigma-Aldrich Co. LLC