product line
BioReagent
assay
≥90% (HPLC), ≥90% (degree of coupling)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in methanol: water (1:1) (with 0.1% perchloric acid)
UV absorption
λ: 532-538 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
General description
Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser.
Application
Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.
Features and Benefits
Characteristic features of the Atto 532 NHS ester are:
- Strong Absorption.
- High Fluorescence quantum yield.
- High Photostability.
- Excellent water solubility.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Rumelo Amor et al.
Scientific reports, 4, 7359-7359 (2014-12-09)
Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot laser scanning microscope by placing a
Stefan Bretschneider et al.
Physical review letters, 98(21), 218103-218103 (2007-08-07)
We report the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by transiently shelving the fluorophore in a metastable dark state. Using a relatively modest light intensity of several kW/cm(2) in a focal distribution featuring a local zero
SERRS-based detection of dye-labeled DNA using positively-charged Ag nanoparticles.
Gill, R. and Lucassen, G. W.
Analytical Methods : Advancing Methods and Applications, 2, 445-447 (2010)
Single-particle and ensemble diffusivities--test of ergodicity.
Florian Feil et al.
Angewandte Chemie (International ed. in English), 51(5), 1152-1155 (2011-10-19)
Toshiro Saito et al.
Nanotechnology, 22(44), 445708-445708 (2011-10-13)
We fabricated platinum bowtie nanostructure arrays producing fluorescence enhancement and evaluated their performance using two-photon photoluminescence and single-molecule fluorescence measurements. A comprehensive selection of suitable materials was explored by electromagnetic simulation and Pt was chosen as the plasmonic material for
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