94506
四甲基罗丹明-5-马来酰亚胺
suitable for fluorescence, ≥85% (HPLC)
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关于此项目
经验公式（希尔记法）:
C28H23N3O5
化学文摘社编号:
分子量:
481.50
UNSPSC Code:
12352116
NACRES:
NA.32
MDL number:
产品名称
四甲基罗丹明-5-马来酰亚胺, suitable for fluorescence, ≥85% (HPLC)
SMILES string
N5(C(=O)C=CC5=O)c1cc(c(cc1)C2=C3CC=C(C=C3Oc4c2ccc(c4)N(C)C)N(C)C)C(=O)O
InChI
1S/C28H25N3O5/c1-29(2)16-5-9-20-23(14-16)36-24-15-17(30(3)4)6-10-21(24)27(20)19-8-7-18(13-22(19)28(34)35)31-25(32)11-12-26(31)33/h5-9,11-15H,10H2,1-4H3,(H,34,35)
InChI key
KGFLZYXDJDOIEE-UHFFFAOYSA-N
assay
≥85% (HPLC)
form
powder
solubility
DMSO: soluble
methanol: soluble
fluorescence
λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
Application
使用四甲基罗丹明-5-马来酰亚胺：
- 浓缩洗脱液并进行标记创建IRE1LDS112C
- 利用外源荧光探针在实验用Cys48上衍生绿色荧光蛋白(GFP)
- 培养单溶剂显露的半胱氨酸突变以进行蛋白质标记
Other Notes
用作细胞荧光分析中蛋白质硫醇的标记试剂
Packaging
无底玻璃瓶。内容物在插入式融锥内。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Fanning SW, et al.,
Nature Communications, 9(1), 2368-2368 (2018)
Cytofluorescence techniques for the visualization of distinct pools of protein thiols at the single cell level.
Alfonso Pompella et al.
Methods in molecular biology (Clifton, N.J.), 196, 305-312 (2002-08-03)
Effect of the point mutation H148G on GFPmut2 unfolding kinetics by fluorescence spectroscopy
Bosisio C, et al.
Biophysical Chemistry, 157(1-3), 24-32 (2011)
A J-protein co-chaperone recruits BiP to monomerize IRE1 and repress the unfolded protein response
Amin-WN, et al.
Cell, 171(7), 1625-1637 (2017)
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