A4503
牛血清白蛋白 来源于牛血清
cold ethanol fraction, pH 5.2, ≥96%
别名:
BSA, 牛白蛋白, 牛血清白蛋白
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥96%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, blood typing: suitable, microbiological culture: suitable
Impurities:
BT and VSV virus, tested
biological source
bovine
Quality Segment
assay
≥96%
form
lyophilized powder
purified by
cold ethanol fractionation
origin
USA origin
technique(s)
ELISA: suitable, blood typing: suitable, microbiological culture: suitable
impurities
BT and VSV virus, tested
loss
≤5%
pH
5.2
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected, VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
Application
- Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
- It has been used in the cytotoxicity assay buffer.
- It has been used for the induction of protein overload nephropathy in rats.
Biochem/physiol Actions
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Preparation Note
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
通常称为 Cohn fraction V；本品采用改进的 Cohn 氏冷乙醇分级分离法制备。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)