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A4503

牛血清白蛋白来源于牛血清

Name: 牛血清白蛋白 来源于牛血清
Brand: SIGMA

cold ethanol fraction, pH 5.2, ≥96%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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关于此项目

化学文摘社编号:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥96%

Form:

lyophilized powder

Technique(s):

ELISA: suitable, blood typing: suitable, microbiological culture: suitable

Impurities:

BT and VSV virus, tested

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属性

biological source

bovine

Quality Level

300

assay

≥96%

form

lyophilized powder

purified by

cold ethanol fractionation

origin

USA origin

technique(s)

ELISA: suitable, blood typing: suitable, microbiological culture: suitable

impurities

BT and VSV virus, tested

loss

≤5%

pH

5.2

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected, VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

说明

General description

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Application

Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
It has been used in the cytotoxicity assay buffer.
It has been used for the induction of protein overload nephropathy in rats.

Biochem/physiol Actions

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

Preparation Note

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。更多的纯化步骤包括结晶或木炭过滤。

通常称为 Cohn fraction V；本品采用改进的 Cohn 氏冷乙醇分级分离法制备。

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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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实验方案

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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Albumin in Cell Culture

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

白蛋白在细胞培养中的应用

本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能，并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。

全球贸易项目编号

货号	GTIN
A4503-50G	04061835553402
A4503-5KG	04061833458358
A4503-1KG	04061835553389
A4503-100G	04061835517619
A4503-10KG	04061833693216
A4503-10G	04061835504480
A4503-500G	04061835553396
A4503-5G	04061833693223