Merck
CN
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文件

安全信息

Y1375

Millipore

YPD肉汤

suitable for microbiology, NutriSelect® Basic

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别名:
酵母菌蛋白胨葡萄糖肉汤
NACRES:
NA.85

无菌性

non-sterile

形式

powder

组成

Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

包装

poly bottle of 1 kg
poly bottle of 250 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

颜色

beige

pH值（酸碱度）

6.3-6.7

application(s)

environmental
food and beverages
microbiology

适用性

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

相关类别

一般描述

YPD肉汤是一种营养通用的液体生长培养基，常用来维持和繁殖毕赤酵母、酿酒酵母和其他酵母。它是一种用于分子克隆、蛋白质表达或富集的培养基。动物组织的消化提供氮营养。酵母提取物提供氮营养物质以及维生素B复合物。葡萄糖为酿酒酵母的生长提供碳水化合物和能量来源。

应用

YPD肉汤推荐用于酿酒酵母菌的生长，以实现分子生物学目的。这种培养基支持大多数异养微生物的生长，但由于其组成简单，一直被用作酵母常规培养的基础培养基。酵母遗传学的一般方法采用酵母提取物蛋白胨-葡萄糖(YPD)培养基培养酿酒酵母及其他酵母。

数量

50 g/L

制备说明

1.取50 g溶于1L蒸馏水中。
2.在121°C下高压灭菌15分钟。

附注

我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。
商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基

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Runhui Liu et al.
Journal of the American Chemical Society, 136(11), 4333-4342 (2014-03-13)
Fungal infections are a major challenge to human health that is heightened by pathogen resistance to current therapeutic agents. Previously, we were inspired by host-defense peptides to develop nylon-3 polymers (poly-β-peptides) that are toxic toward the fungal pathogen Candida albicans
Christina Bergonzo et al.
Proteins, 88(9), 1189-1196 (2020-03-18)
ClpS2 is a small protein under development as a probe for selectively recognizing N-terminal amino acids of N-degron peptide fragments. To understand the structural basis of ClpS2 specificity for an N-terminal amino acid, all atom molecular dynamics (MD) simulations were
Veronique Archambault-Leger et al.
Biotechnology for biofuels, 5(1), 49-49 (2012-07-21)
The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two conversion systems (simultaneous saccharification and fermentation using yeast and fungal cellulase, and fermentation
Julia Kennedy-Darling et al.
Analytical chemistry, 86(12), 5678-5681 (2014-05-23)
Protein-DNA binding interactions play critical roles in important cellular processes such as gene expression, cell division, and chromosomal organization. Techniques to identify and characterize these interactions often utilize formaldehyde cross-linking for stabilization of the complexes. Advantages of formaldehyde as a
Anne Kerlikowski et al.
Journal of biophotonics, 13(12), e202000221-e202000221 (2020-09-16)
Reinfection in endodontically treated teeth is linked to the complexity of the root canal system, which is problematic to reach with conventional disinfection methods. As plasma is expected to have the ability to sanitize narrow areas, the aim of this
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Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

蛋白质表达系统

基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。

实验方案

酵母生长实验方案

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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