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安全信息

YEAST1

Sigma-Aldrich

酵母转化试剂盒

reagents for introducing plasmid DNA into yeast

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别名:
lithium acetate yeast transformation
NACRES:
NA.85

等级

for molecular biology

质量水平

200

用途

 kit sufficient for >100 standard transformations

technique(s)

transformation: suitable

运输

dry ice

储存温度

−20°C

一般描述

Sigma 的酵母转化试剂盒包含了通过醋酸锂方法高效转化酵母的所有必要试剂和对照品。

应用

适用于任何酵母菌株的转化。 方便灵活灵敏，只需 10 ng 的 DNA 即可获得阳性转化子；最佳效率在 0.1-3μg 范围。

特点和优势

  • 简单易用
  • 只需 10 ng 质粒 DNA
  • 任何酵母菌株的灵活性
  • 足以进行超过 100 次标准转换

组分

酵母转化试剂盒包含：
  • 转化缓冲液；100 ml；100 mM 醋酸锂、10 mM Tris HCl (pH 7.6) 和 1 mM EDTA
  • 平板缓冲液；100 mL；40%PEG，100 mM 醋酸锂，10 mM Tris HCl，pH 7.5，1 mM EDTA
  • 鲑鱼试验的脱氧核糖核酸，10 mg/mL；2 x 1 mL
  • 携带 ura 基因的对照酵母质粒 DNA pRS316；10 μg
  • 不含尿嘧啶的酵母合成脱落培养基补充剂；1 g

原理

用补充突变基因的质粒转化使转化子在缺乏所需成分的培养基上生长。通过在缓冲醋酸锂溶液中孵育，使酵母细胞具有转化能力。然后在含聚乙二醇 (PEG) 的溶液中将细胞与转化 DNA 和载体 DNA 一起孵育进行转化。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1化学品安全说明书
  • Y1501Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g化学品安全说明书

相关产品

产品编号
说明
价格

D8418

二甲基亚砜, for molecular biology

A5306

细菌琼脂, for molecular biology

G5516

甘油, for molecular biology, ≥99.0%

Y1625

酵母提取物, for use in microbial growth medium

Y0750

酵母营养缺陷型培养基补充剂, without leucine and tryptophan

Y1751

酵母营养缺陷型培养基补充剂, without histidine

Y2001

酵母营养缺陷型培养基补充剂, without histidine, leucine, tryptophan and uracil

Y1376

酵母营养缺陷型培养基补充剂, without leucine

Y0626

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Y1876

酵母营养缺陷型培养基补充剂, without tryptophan

象形图

Health hazard
GHS08

警示用语：

Warning

危险声明

H373

预防措施声明

P260 - P314 - P501

危险分类

STOT RE 2 Inhalation

靶器官

Respiratory Tract

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Christoph Sygmund et al.
Microbial cell factories, 12, 38-38 (2013-04-27)
The ability of fungal cellobiose dehydrogenase (CDH) to generate H2O2 in-situ is highly interesting for biotechnological applications like cotton bleaching, laundry detergents or antimicrobial functionalization of medical devices. CDH's ability to directly use polysaccharide derived mono- and oligosaccharides as substrates
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While the Pycnoporus cinnabarinus laccase (PcL) is one of the most promising high-redox-potential enzymes for environmental biocatalysis, its practical use has to date remained limited due to the lack of directed evolution platforms with which to improve its features. Here
DMSO-enhanced whole cell yeast transformation.
J Hill et al.
Nucleic acids research, 19(20), 5791-5791 (1991-10-25)
James R Petrie et al.
PloS one, 7(4), e35214-e35214 (2012-04-24)
Monoacylglycerol acyltransferases (MGATs) are predominantly associated with lipid absorption and resynthesis in the animal intestine where they catalyse the first step in the monoacylglycerol (MAG) pathway by acylating MAG to form diacylglycerol (DAG). Typical plant triacylglycerol (TAG) biosynthesis routes such
Francesco Palma et al.
FEMS microbiology letters, 272(1), 114-119 (2007-06-22)
The TBF-1 is an 11.9-kDa fruiting body specific protein of the Ascomycetes hypogeous fungus Tuber borchii Vittad. found in aqueous extract and the hyphal cell wall. The tbf-1 gene codes a 12-amino acid N-terminal stretch not present in mature protein.
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Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

酵母转化简介

转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。

蛋白质表达系统

基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

实验方案

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

酵母转化实验方法

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

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