跳转至内容
Merck
CN

YEAST1

酵母转化试剂盒

reagents for introducing plasmid DNA into yeast

别名:

lithium acetate yeast transformation

登录 查看组织和合同定价。

选择尺寸


关于此项目

NACRES:
NA.85
UNSPSC Code:
12352200
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

酵母转化试剂盒, reagents for introducing plasmid DNA into yeast

grade

Molecular Biology

technique(s)

transformation: suitable

shipped in

dry ice

storage temp.

−20°C

Quality Level

usage

 kit sufficient for >100 standard transformations

Application

适用于任何酵母菌株的转化。 方便灵活灵敏,只需 10 ng 的 DNA 即可获得阳性转化子;最佳效率在 0.1-3μg 范围。

Biochem/physiol Actions

用补充突变基因的质粒转化使转化子在缺乏所需成分的培养基上生长。通过在缓冲醋酸锂溶液中孵育,使酵母细胞具有转化能力。然后在含聚乙二醇 (PEG) 的溶液中将细胞与转化 DNA 和载体 DNA 一起孵育进行转化。

Features and Benefits

  • 简单易用
  • 只需 10 ng 质粒 DNA
  • 任何酵母菌株的灵活性
  • 足以进行超过 100 次标准转换

General description

Sigma 的酵母转化试剂盒包含了通过醋酸锂方法高效转化酵母的所有必要试剂和对照品。

Other Notes

酵母转化试剂盒包含:
  • 转化缓冲液;100 ml;100 mM 醋酸锂、10 mM Tris HCl (pH 7.6) 和 1 mM EDTA
  • 平板缓冲液;100 mL;40%PEG,100 mM 醋酸锂,10 mM Tris HCl,pH 7.5,1 mM EDTA
  • 鲑鱼试验的脱氧核糖核酸,10 mg/mL;2 x 1 mL
  • 携带 ura 基因的对照酵母质粒 DNA pRS316;10 μg
  • 不含尿嘧啶的酵母合成脱落培养基补充剂;1 g

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Tim R Blower et al.
Nucleic acids research, 47(15), 8163-8179 (2019-07-10)
Type II topoisomerases catalyze essential DNA transactions and are proven drug targets. Drug discrimination by prokaryotic and eukaryotic topoisomerases is vital to therapeutic utility, but is poorly understood. We developed a next-generation sequencing (NGS) approach to identify drug-resistance mutations in
Improved method for high efficiency transformation of intact yeast cells.
D Gietz et al.
Nucleic acids research, 20(6), 1425-1425 (1992-03-25)
Miren Zumárraga et al.
Chemistry & biology, 14(9), 1052-1064 (2007-09-22)
Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in
A simple and efficient procedure for transformation of yeasts.
R Elble
BioTechniques, 13(1), 18-20 (1992-07-01)
Widening the pH activity profile of a fungal laccase by directed evolution.
Torres-Salas P
Chembiochem, 14(8), 934-937 (2013)

商品

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

转化是将外源DNA引入细胞,引起遗传变异或基因修饰的过程。1928年,Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。

基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。

实验方案

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。

相关内容

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持