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Merck
CN

A1047

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

别名:

Goat Anti-Mouse IgG (γ-chain specific)−AP

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
direct ELISA
western blot
Species reactivity:
mouse
Citations:
9
Technique(s):
direct ELISA: 1:7,000-1:21,000
western blot: suitable
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产品名称

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous solution

biological source

goat

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:7,000-1:21,000
western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

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Application

Antibodies to Trypanosoma cruzi were detected in the sera of infected mice by ELISA with an alkaline phosphatase conjugated goat anti-mouse IgG chain specific. Plates were coated with T. cruzi antigen and blocked with 1% BSA/PBS prior to incubation with conjugated antibody.
For detection of IgG antibodies in mouse sera and saliva following S. gordonii infection, ELISA assays were performed using anti-mouse IgG alkaline phosphatase antibody at 1:20000. This antibody did not detect IgA antibodies.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibodies can be used for western blot assays,.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibody associates with mouse IgG (gamma-chain specific).

Immunogen

purified mouse IgG

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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分析证书(COA)

Lot/Batch Number

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Dieter Palmberger et al.
Biotechnology journal, 9(9), 1206-1214 (2014-04-23)
The baculovirus/insect cell system has proven to be a very powerful tool for the expression of several therapeutics. Nevertheless, these products sometimes suffer from reduced biological activity and unwanted side effects. Several studies have demonstrated that glycosylation can greatly influence
Florian Krammer et al.
Journal of virological methods, 167(1), 17-22 (2010-03-23)
Various virus-like particles (VLPs) have been shown to induce cytotoxic T-cell immune response as well as B-cell immune response. This makes VLPs promising candidates for antigen-carrier platforms for various epitopes. Influenza A VLPs were produced displaying a 20 amino acid
J Klingström et al.
Journal of medical virology, 68(4), 581-588 (2002-10-12)
In order to investigate rodent host specificity of European hantaviruses, experimental infection of colonized and wild-trapped rodents was performed. In addition to the natural rodent reservoir, Clethrionomys glareolus, Puumala hantavirus (PUUV) could infect colonized Microtus agrestis and Lemmus sibiricus, but
R A Clarke et al.
International journal of radiation oncology, biology, physics, 41(5), 1021-1027 (1998-08-27)
Severe acute toxicity limits the effective use of radiotherapy in patients who are radiosensitive, and it is not usually possible to identify these radiohypersensitive (R-H) individuals before treatment commences. Five such R-H patients were detected over a 3-year period. We
Lu Fan et al.
ACS omega, 7(33), 28932-28945 (2022-08-30)
While many proteins are known clients of heat shock protein 90 (Hsp90), it is unclear whether the transcription factor, thyroid hormone receptor beta (TRb), interacts with Hsp90 to control hormonal perception and signaling. Higher Hsp90 expression in mouse fibroblasts was

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