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Merck
CN

A1054

抗-地高辛-碱性磷酸酶抗体,小鼠单克隆

clone DI-22, purified from hybridoma cell culture

别名:

单克隆抗-地高辛, 单克隆抗 地高辛 – 碱性磷酸酶 小鼠抗

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关于此项目

NACRES:
NA.46
UNSPSC Code:
12352203
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产品名称

抗-地高辛-碱性磷酸酶抗体,小鼠单克隆, clone DI-22, purified from hybridoma cell culture

biological source

mouse

recombinant

expressed in mouse cell line

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DI-22, monoclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate
direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

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Application

巢式PCR产物可与5′端地高辛标记的寡核苷酸探针杂交。 向1 M磷酸盐缓冲液、1%牛血清白蛋白和0.1%吐温20(pH 7.4)中添加1:1000稀释的抗地高辛单克隆抗体碱性磷酸酶(DI-22)进行ELISA分析,以检测探针。使用Sigma Fass-Pnpp底物片剂进行检测。
抗地高辛碱性磷酸酶单克隆抗体适用于直接ELISA(1:10000-1:6000)和斑点杂交(以1:20,000稀释,使用地高辛标记的生物分子)。它还用于酶免疫分析(EIA)。

Biochem/physiol Actions

抗体对地高辛具有特异性,且对地高辛具有高亲和力。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

地高辛是一种强心苷,可用于治疗慢性心力衰竭。抗地高辛碱性磷酸酶单克隆抗体可用于检测地高辛标记的化合物,例如寡核苷酸、抗体和肽。该抗体对地高辛和地高辛标记的化合物具有特异性。该产品与地高辛具有很强的交叉反应性。

Immunogen

地高辛-KLH。

Physical form

0.05 M Tris缓冲液,pH 8.0,含1% BSA、50%甘油、1 mM MgCl2和15 mM叠氮化钠

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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