A1231
Monoclonal Anti-Aurora-A Kinase 小鼠抗
clone 35C1, purified from hybridoma cell culture
别名:
Anti-ARK-1, Anti-AURORA2, Anti-Aurora-related kinase 1, Anti-STK6, Anti-Serine/threonine protein kinase 15, AntiSTK15 (Serine/threonine protein kinase 15), BTAK
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关于此项目
Conjugate:
unconjugated
Clone:
35C1, monoclonal
Application:
immunocytochemistry
immunoprecipitation (IP)
indirect ELISA
western blot
immunoprecipitation (IP)
indirect ELISA
western blot
Species reactivity:
human, mouse
Citations:
20
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using HeLa total cell extract
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using HeLa total cell extract
Uniprot accession no.:
产品名称
Monoclonal Anti-Aurora-A Kinase 小鼠抗, clone 35C1, purified from hybridoma cell culture
biological source
mouse
recombinant
expressed in mouse cell line
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
35C1, monoclonal
form
PBS solution
mol wt
antigen ~46 kDa
species reactivity
human, mouse
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using HeLa total cell extract
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... AURKA(6790), AURKA(6790)
mouse ... Aurka(20878), Aurka(20878)
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Application
Anti-Aurora-A Kinase antibody, Mouse monoclonal has been used in immunohistochemistry.
Monoclonal Anti-Aurora-A Kinase antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot, indirect ELISA and western blot (0.25-0.5 μg/mL using HeLa total cell extract). The antibody product does not inhibit kinase activity of Aurora-A kinase, hence the product can be used to measure the in vivo aurora-A kinase activity after immunoprecipitation.
Biochem/physiol Actions
Aurora-A (Aur-A) kinase is a serine threonine kinase that regulates cell division. Elevated levels of Aur-A have been associated with inhibition of apoptosis, and evasion of spindle assembly checkpoints. Aur-A may also be used as a prognostic marker for nasopharyngeal cancers in humans .
Aurora-A kinase is involved in centrosome separation, centrosome maturation, bipolar spindle assembly and stability. Monopolar spindles are formed in the cell when the expression of aAurora-A kinase is inhibited by an appropriate siRNA, while over-expression of this protein results in centrosome amplification and polyploidy of the cell because of cytokinesis failure. Aurora-A activity is essential for the recruitment of cyclin dependent kinase (CDK)-cyclin B1 to the centrosome, which relates with its activation and the commitment of the cells to mitosis. Activation of CDK1 kinase is by dephosphorylation of Tyr15 by CDC25B. Aurora-A kinase phosphorylates CDC25B both in vitro and in vivo at Ser353. This phosphorylation occurs at the centrosome during progression from prophase to anaphase. This regulation is important for entry into mitosis.
Monoclonal Anti-Aurora-A Kinase antibody is specific for Aurora-A Kinase in humans and mice.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Aurora-A Kinase antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 35C1 produced by the fusion of mouse myeloma cells (SP2/0-Ag14) and splenocytes from BALB/c mice immunized with recombinant human Aurora-A Kinase. Aurora-A Kinase is encoded by serine/threonine kinase (STK15) gene. During S-phase to mitosis progression in cell cycle, the protein is located at the centrosome.
Immunogen
recombinant human Aurora-A kinase.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Aurora-A Identifies Early Recurrence and Poor Prognosis and Promises a Potential Therapeutic Target in Triple Negative Breast Cancer
Xu J, et al.
PLoS ONE, 8(2), e56919-e56919 (2013)
Qian He et al.
Molecular biology of the cell, 25(11), 1715-1729 (2014-04-04)
We show here that human embryonic stem (ES) and induced pluripotent stem cell-derived neuroprogenitors (NPs) develop primary cilia. Ciliogenesis depends on the sphingolipid ceramide and its interaction with atypical PKC (aPKC), both of which distribute to the primary cilium and
Shulan Sun et al.
Cancer communications (London, England), 41(9), 851-866 (2021-07-13)
Increasing studies have reported that oncogenes regulate components of the immune system, suggesting that this is a mechanism for tumorigenesis. Aurora kinase A (AURKA), a serine/threonine kinase, is involved in cell mitosis and is essential for tumor cell proliferation, metastasis
Edward J Morris et al.
Nature communications, 8, 15289-15289 (2017-05-06)
Cancer cells frequently have amplified centrosomes that must be clustered together to form a bipolar mitotic spindle, and targeting centrosome clustering is considered a promising therapeutic strategy. A high-content chemical screen for inhibitors of centrosome clustering identified Stattic, a Stat3
Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2M transition
Dutertre S, et al.
Journal of Cell Science, 117(12), 2523-2531 (2004)
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