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Merck
CN

A1543

抗 人 IgG(全分子)-碱性磷酸酶 山羊抗

IgG fraction of antiserum, buffered aqueous solution

别名:

Goat Anti-Human IgG (whole molecule)−AP

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p), WB CL
Citations:
18
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biological source

goat

Quality Level

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:20,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot (chemiluminescent): 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

人IgG是糖蛋白抗体,含有两条相等的轻链和一对相同的重链,其分子量分别为 50kDa 和 25kDa。IgG具有四种不同亚型,范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。山羊抗人IgG与人IgG结合。免疫球蛋白G(IgG)属于免疫球蛋白家族,是一种广泛表达的血清抗体。该抗体的一级结构还包含连接两条重链、连接重链和轻链,并位于链内的二硫键。

Immunogen

从人血清中纯化的 IgG

Application

人血清IgG水平通过固相ELISA测定,即使用碱性磷酸酶结合的山羊抗人IgG抗体,在37°C下培育1小时。该实验使用0.1%对硝基苯磷酸酯(Sigma)作为底物进行测定。通过蛋白质印迹分析,使用稀释比例为1:1000的碱性磷酸酶结合山羊抗人IgG抗体,检测人血清中对汉城病毒(80/39株)的抗体。采用氮蓝四唑和5-溴-4-氯-3-吲哚-磷酸酯底物(Sigma)进行印迹。
羊抗人 IgG 也可用于斑点印迹 (1:30000) 和免疫组化 (1:50) 应用。

Physical form

0.05 MTris (pH 8.0) 溶液,含 1% 牛血清白蛋白、1 mM MgCl 2 和 15 mM 叠氮化钠

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
低风险生物材料

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分析证书(COA)

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Heetae Lee et al.
Journal of medical virology, 84(12), 1943-1952 (2012-10-20)
A highly significant increase in anti-Vesivirus (family Caliciviridae) antibody prevalence, along the axis from healthy blood donors; donors with elevated transaminase; patients with clinical hepatitis; and patients with post-transfusion/dialysis hepatitis, has been reported in human sera from the USA and
Sophana Chea et al.
Frontiers in immunology, 15, 1368066-1368066 (2024-05-16)
Aedes spp. are the most prolific mosquito vectors in the world. Found on every continent, they can effectively transmit various arboviruses, including the dengue virus which continues to cause outbreaks worldwide and is spreading into previously non-endemic areas. The lack
Jessica E Manning et al.
The Journal of infectious diseases, 226(8), 1327-1337 (2021-11-01)
We established the first prospective cohort to understand how infection with dengue virus is influenced by vector-specific determinants such as humoral immunity to Aedes aegypti salivary proteins. Children aged 2-9 years were enrolled in the PAGODAS (Pediatric Assessment Group of
L Lledó et al.
Journal of medical microbiology, 51(10), 861-865 (2002-11-19)
Data relating to hantavirus infection in Spain are scarce and limited to rural areas. The aim of this work was to study the seroprevalence of hantavirus infection in the Autonomous Community of Madrid (ACM), a region containing both rural and
Jens Bukh et al.
Journal of medical virology, 80(1), 87-94 (2007-11-28)
GB virus-B (GBV-B) is the virus most closely related to hepatitis C virus (HCV). Thus, we have used GBV-B infection of tamarins, which develop acute hepatitis following experimental infection, as a surrogate model to study protective immunity. As challenge virus

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全球贸易项目编号

货号GTIN
A1543-1ML04061833342442

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