biological source
goat
Quality Level
recombinant
expressed in goat
conjugate
alkaline phosphatase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:20,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot (chemiluminescent): 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
人IgG是糖蛋白抗体,含有两条相等的轻链和一对相同的重链,其分子量分别为 50kDa 和 25kDa。IgG具有四种不同亚型,范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。山羊抗人IgG与人IgG结合。免疫球蛋白G(IgG)属于免疫球蛋白家族,是一种广泛表达的血清抗体。该抗体的一级结构还包含连接两条重链、连接重链和轻链,并位于链内的二硫键。
Immunogen
从人血清中纯化的 IgG
Application
人血清IgG水平通过固相ELISA测定,即使用碱性磷酸酶结合的山羊抗人IgG抗体,在37°C下培育1小时。该实验使用0.1%对硝基苯磷酸酯(Sigma)作为底物进行测定。通过蛋白质印迹分析,使用稀释比例为1:1000的碱性磷酸酶结合山羊抗人IgG抗体,检测人血清中对汉城病毒(80/39株)的抗体。采用氮蓝四唑和5-溴-4-氯-3-吲哚-磷酸酯底物(Sigma)进行印迹。
羊抗人 IgG 也可用于斑点印迹 (1:30000) 和免疫组化 (1:50) 应用。
Physical form
0.05 MTris (pH 8.0) 溶液,含 1% 牛血清白蛋白、1 mM MgCl 2 和 15 mM 叠氮化钠
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
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相关内容
抗体的质量决定了整个免疫检测的成败, 是在选择抗体时首选的考虑因素。基于Sigma®、Chemicon®、Upstate®、Mil l ipore®、 Calbiochem®和Novagen®等子品牌的强强联合,默克在创新的 免疫原设计、免疫、挑选、筛选和验证等方面有着数十年的经验, 创造出许多被广泛使用的抗体,如4G10,Neun,组蛋白修饰等抗 体都是相关领域的“金标”抗体。 我们的技术和科研背景使默克成为高质量抗体的主要设计者和生产 者,而不仅仅是经销商。毋庸置疑,我们的每个小瓶中的抗体都包 含着大量的科学研究成果。
Datasheet
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A1543-1ML | 04061833342442 |
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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