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A2153

牛血清白蛋白来源于牛血清

Name: 牛血清白蛋白 来源于牛血清
Brand: SIGMA

lyophilized powder, ≥96% (agarose gel electrophoresis)

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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关于此项目

化学文摘社编号:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥96% (agarose gel electrophoresis)

Form:

lyophilized powder

Technique(s):

ELISA: suitable, blood typing: suitable, microbiological culture: suitable

Impurities:

≤6.0% Loss on Drying

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biological source

bovine

Quality Level

200

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable, blood typing: suitable, microbiological culture: suitable

impurities

≤6.0% Loss on Drying

loss

≤6%

pH

6.5-7.5 (1% in 0.15 M sodium chloride), 7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected, VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

牛血清白蛋白 (BSA) 是牛肝脏产生的一种球状非糖基化α-螺旋血浆蛋白。 它含有三种结构域（I，II，III），每个结构域都含有两个心形结构的亚结构域（A和B）。

Application

牛血清白蛋白已用于：

在精子运动分析中与BTS (贝兹维尔解冻液)一起用于拉伸精子
作为改良 Krebs-Ringer缓冲液 (dmKRBT)的成分
凝胶迁移分析
作为免疫细胞化学、免疫组织化学、(4) 免疫荧光实验和夹心酶联免疫吸附分析(ELISA)中的封闭剂
在流式细胞术中作为磷酸盐缓冲液 (PBS) 的成分用于重悬细胞进行分析
作为蛋白质标准品用于通过Bradford法定量测定组织样品中的蛋白质
测定17β HSD酶的酶活性

Biochem/physiol Actions

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

Preparation Note

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。更多的纯化步骤包括结晶或木炭过滤。

通常称为第五组分(Cohn fraction V)；本品采用改进的Cohn氏冷乙醇分级分离法制备。

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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全球贸易项目编号

货号	GTIN
A2153-1KG	04061835551149
A2153-5KG	04061833456132
A2153-100G	04061835517466
A2153-10G	04061835551132
A2153-50G	04061835517473
A2153-5G	04061833693124
A2153-10KG	04061833693117
A2153-500G	04061835551156

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