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安全信息

A2153

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

lyophilized powder, ≥96% (agarose gel electrophoresis)

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别名:
BSA, 牛白蛋白, 牛血清白蛋白
CAS号:
9048-46-8
EC 号:
232-936-2
MDL编号:
MFCD00130384
NACRES:
NA.27

生物来源

bovine

质量水平

200

检测方案

≥96% (agarose gel electrophoresis)

形式

lyophilized powder

分子量

~66 kDa

纯化方式

cold ethanol fractionation

包装

poly bottle of

来源

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

杂质

≤6.0% Loss on Drying

缺失

≤6%

pH值（酸碱度）

6.5-7.5 (1% in 0.15 M sodium chloride)
7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

P02769

异质活性

BT Virus, none detected
VSV Virus, none detected

储存温度

2-8°C

Gene Information

bovine ... ALB(280717)

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相关类别

一般描述

牛血清白蛋白 (BSA) 是牛肝脏产生的一种球状非糖基化α-螺旋血浆蛋白。 它含有三种结构域（I，II，III），每个结构域都含有两个心形结构的亚结构域（A和B）。

应用

牛血清白蛋白已用于：

  • 在精子运动分析中与BTS (贝兹维尔解冻液)一起用于拉伸精子
  • 作为改良 Krebs-Ringer缓冲液 (dmKRBT)的成分
  • 凝胶迁移分析
  • 作为免疫细胞化学、免疫组织化学、(4) 免疫荧光实验和夹心酶联免疫吸附分析(ELISA)中的封闭剂
  • 在流式细胞术中作为磷酸盐缓冲液 (PBS) 的成分用于重悬细胞进行分析
  • 作为蛋白质标准品用于通过Bradford法定量测定组织样品中的蛋白质
  • 测定17β HSD酶的酶活性

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
通常称为第五组分(Cohn fraction V)；本品采用改进的Cohn氏冷乙醇分级分离法制备。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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  1. Is Product A2153, Albumin from bovine serum (BSA), suitable for use in ELISA?

    We have not tested A2153 in quality control for use in ELISA applications. Because it contains globulins, we would generally not recommend A2153 for use in ELISA.

  2. Is Product A2153, Albumin from bovine serum (BSA), suitable for use in immunoblotting experiments as a blocking agent?

    We have not tested A2153 in quality control for use in immunoblotting applications as a blocking agent. Because it contains globulins, we would generally not recommend A2153 for use as a blocking agent.

  3. Product A2153, Albumin from bovine serum (BSA), powder looks tan in color.  Is this OK?

    The tan color is within specifications. The color can vary because of factors such as concentration of a solution at the time of lyophilization.

  4. Is Product A2153, Albumin from bovine serum (BSA), suitable for cell culture?

    We do not test A2153 for cell culture use, or for any specifications such as endotoxin content. Customers will have to exercise their own judgment as to whether they wish to use A2153 in cell culture, or can search the literature for publications that cite this product number.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Simple histochemical stain for acrosomes on sperm from several species.
Larson JL and Miller DJ
Molecular Reproduction and Development, 52(4), 445-449 (1999)
Bartolomeo Della Ventura et al.
PloS one, 12(2), e0171754-e0171754 (2017-02-10)
Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of the contaminants in water as well as solid matrices. Although important to be detected, small analytes (few hundreds of Daltons) are an issue
Mateusz Majda et al.
Developmental cell, 43(3), 290-304 (2017-11-08)
The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result
Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry.
Guthrie HD and Welch GR
Journal of Animal Science, 84(8), 2089-2100 (2006)
Maleeha F Ahmad et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(2), 188-199 (2018-12-21)
Objective- Perivascular adipose tissue (PVAT) contains an independent adrenergic system that can take up, metabolize, release, and potentially synthesize the vasoactive catecholamine norepinephrine. Norepinephrine has been detected in PVAT, but the mechanism of its protection within this tissue is unknown.
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