产品名称
抗-人IgG(γ-链特异性),F(ab′)2 片段−过氧化物酶 山羊抗, affinity isolated antibody, buffered aqueous solution
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
direct ELISA: 1:10,000
western blot: suitable
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Preparation Note
按照Wilson, M.B.,和Nakane, P.K.在由阿姆斯特丹Elsevier/北荷兰生物医学出版社出版的免疫荧光及相关染色技术(1978)一书中所描述的高碘酸盐法而制备。
Application
成功使用该抗体的应用以及相关的同行评审论文如下。
酶联免疫吸附试验(1篇)
酶联免疫吸附试验(1篇)
抗人IgG(γ-链特异性),(F(ab′)2)片段-过氧化物酶抗体适用于ELISA和蛋白质印迹。
过氧化物酶缀合的山羊抗人IgG(Fab特异性)抗体可用于遗传修饰的酵母培养物的蛋白质印迹和ELISA分析,以产生重组Fab片段。在PBS中以1:10,000稀释抗人IgG抗体,并在室温下温育1小时。使用O-亚苯基-二胺底物溶液(Sigma)对ELISA测定中的结合进行检测。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
人IgG是糖蛋白抗体,含有两条相等的轻链和一对相同的重链。 IgG具有四种不同亚型,范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。当针对人IgA、IgG、IgM、本周氏蛋白κ和λ骨髓瘤蛋白进行测试时,抗人IgG(γ-链特异性),(F(ab′)2)片段过氧化物酶抗体对人IgG具有特异性。
Immunogen
纯化人IgG
Physical form
0.01 M磷酸盐缓冲液(pH 7.4)中的溶液,含有1%牛血清白蛋白和防腐剂
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
此项目有
L D Ballam et al.
Journal of clinical pathology, 53(4), 314-317 (2000-05-24)
The salivary diagnosis of Helicobacter pylori infection offers attractive possibilities for the epidemiological study of infection in children. Salivary enzyme linked immunosorbent assay (ELISA) is less reliable then serum ELISA, owing to variable transudation of immunoglobulin. In addition, children are
Joanne L Zahorsky-Reeves et al.
BMC immunology, 7, 3-3 (2006-03-22)
The use of porcine cells and organs as a source of xenografts for human patients would vastly increase the donor pool; however, both humans and Old World primates vigorously reject pig tissues due to xenoantibodies that react with the polysaccharide
Joanne L Zahorsky-Reeves et al.
Xenotransplantation, 14(2), 135-144 (2007-03-27)
Recent work has indicated a role for anti-Gal alpha 1-3Gal (Gal) and anti-non-Gal xenoantibodies in the primate humoral rejection response against human-decay accelerating factor (hDAF) transgenic pig organs. Our laboratory has shown that anti-porcine xenograft antibodies in humans and non-human
Philippe Gatault et al.
mAbs, 7(6), 1205-1211 (2015-09-05)
The annual cost of eculizumab maintenance therapy in paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic-uremic syndrome (aHUS) exceeds $300,000 per patient. A better understanding of eculizumab pharmacokinetics and subsequent individual dose adjustment could reduce this cost. We measured the trough
Nils Mülling et al.
Infection (2023-05-27)
Vaccination against Streptococcus pneumoniae is recommended in transplant recipients to reduce the morbidity and mortality from invasive pneumococcal disease. Previous studies indicate that transplant recipients can produce specific antibodies after vaccination with the 13-valent pneumococcal conjugate vaccine Prevenar 13 (PCV13)
相关内容
抗体的质量决定了整个免疫检测的成败, 是在选择抗体时首选的考虑因素。基于Sigma®、Chemicon®、Upstate®、Mil l ipore®、 Calbiochem®和Novagen®等子品牌的强强联合,默克在创新的 免疫原设计、免疫、挑选、筛选和验证等方面有着数十年的经验, 创造出许多被广泛使用的抗体,如4G10,Neun,组蛋白修饰等抗 体都是相关领域的“金标”抗体。 我们的技术和科研背景使默克成为高质量抗体的主要设计者和生产 者,而不仅仅是经销商。毋庸置疑,我们的每个小瓶中的抗体都包 含着大量的科学研究成果。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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