A3054
琼脂糖
For pulsed field electrophoresis sample preparation
别名:
3,6-Anhydro-α-L-galacto-β-D-galactan
生物来源
algae (red)
表单
powder
技术
electrophoresis: suitable
杂质
≤10% water
EEO
≤0.10
转变温度
gel point ≤30 °C
痕量阴离子
sulfate (SO42-): ≤0.15%
异质活性
DNase and RNase, none detected
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
正在寻找类似产品? 访问 产品对比指南
应用
专用于制备染色体 DNA 样品,然后进行脉冲场凝胶电泳 (PFGE)。形成凝胶块用于分离高分子量的 DNA,可以避免传统分离会遇到的剪切。包埋在琼脂糖块中时,细胞可溶解且由限制性内切酶消化 DNA。
分析说明
以下是与我们的琼脂糖相关的性质列表:
硫酸盐含量 - 用作纯度指标,因为硫酸盐是其中存在的主要离子基团。
凝胶强度 - 破裂凝胶所需施加的力。
胶凝点 - 琼脂糖水溶液在冷却时的胶凝温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说,它们的凝胶点与它们的熔点温度不同。
电内渗(EEO)- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动,但可解离的抗衡阳离子可以向基质中的阴极迁移,从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的,因此EEO会因为内部的对流而破坏分离。
硫酸盐含量 - 用作纯度指标,因为硫酸盐是其中存在的主要离子基团。
凝胶强度 - 破裂凝胶所需施加的力。
胶凝点 - 琼脂糖水溶液在冷却时的胶凝温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说,它们的凝胶点与它们的熔点温度不同。
电内渗(EEO)- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动,但可解离的抗衡阳离子可以向基质中的阴极迁移,从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的,因此EEO会因为内部的对流而破坏分离。
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Sujith V W Weerasinghe et al.
Journal of medicinal chemistry, 51(18), 5542-5551 (2008-08-30)
Histone deacetylase 1 (HDAC1) has been linked to cell growth and cell cycle regulation, which makes it a widely recognized target for anticancer drugs. Whereas variations of the metal-binding and capping groups of HDAC inhibitors have been studied extensively, the
Michael J Lafferty et al.
The Journal of biological chemistry, 288(40), 28524-28534 (2013-08-21)
Elevated triglycerides are associated with an increased risk of cardiovascular disease, and lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of triglycerides from circulating lipoproteins. The N-terminal domain of angiopoietin-like protein 4 (ANGPTL4) inhibits LPL activity. ANGPTL4 was
Julia Dahlmann et al.
Biomaterials, 34(10), 2463-2471 (2013-01-22)
In most pluripotent stem cell differentiation protocols the formation of embryoid bodies (EBs) is an important step. Here we describe a rapid, straightforward soft lithography approach for the preparation of hydrophilic silicon masters from different templates and the subsequent production
Markus Landthaler et al.
RNA (New York, N.Y.), 14(12), 2580-2596 (2008-11-04)
microRNAs (miRNAs) regulate the expression of mRNAs in animals and plants through miRNA-containing ribonucleoprotein particles (RNPs). At the core of these miRNA silencing effector complexes are the Argonaute (AGO) proteins that bind miRNAs and mediate target mRNA recognition. We generated
Benjamin Pluvinage et al.
The Journal of biological chemistry, 288(39), 28078-28088 (2013-08-08)
The bacteria that metabolize agarose use multiple enzymes of complementary specificities to hydrolyze the glycosidic linkages in agarose, a linear polymer comprising the repeating disaccharide subunit of neoagarobiose (3,6-anhydro-l-galactose-α-(1,3)-d-galactose) that are β-(1,4)-linked. Here we present the crystal structure of a
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持