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关于此项目
化学文摘社编号:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, western blot: suitable
Impurities:
essentially globulin free, protease, none detected
biological source
bovine
Quality Level
assay
≥98%
form
lyophilized powder
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable, western blot: suitable
impurities
essentially globulin free, protease, none detected
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected, VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
牛血清白蛋白(BSA)在血液胶体渗透压的调节中起重要作用。 BSA 是牛血清中的一种主要蛋白,包含583个氨基酸,计算分子量为66,430道尔顿。BSA被用于许多不同的应用和领域,例如:
这种特殊的BSA经过专门评估和测试,具有非常低的蛋白酶含量/无蛋白酶状态。 不含蛋白酶的BSA可用于以下应用中:
b)核酸杂交
c)放射免疫测定(RIA)
- 蛋白质定量(作为标准品)
- 免疫化学(作为封闭剂)
- 细胞培养(作为营养物质)
这种特殊的BSA经过专门评估和测试,具有非常低的蛋白酶含量/无蛋白酶状态。 不含蛋白酶的BSA可用于以下应用中:
- 酶测定
- 蛋白质测定
- 蛋白酶敏感型技术,包括:
b)核酸杂交
c)放射免疫测定(RIA)
Application
牛血清白蛋白已用于发光分析。
Biochem/physiol Actions
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Features and Benefits
- 热休克分级
- 不含蛋白酶
- 几乎不含球蛋白
Preparation Note
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
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相关内容
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A3059-500G | 04061835553259 |
| A3059-5G | 04061833693155 |
| A3059-10G | 04061835504473 |
| A3059-200MG | 04061833400173 |
| A3059-100G | 04061835553242 |
| A3059-50G | 04061835511686 |
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