A3187
抗人 IgG（γ-链特异性）-碱性磷酸酶 山羊抗
affinity isolated antibody, buffered aqueous glycerol solution
别名:
Goat Anti-Human IgG (γ-chain specific)−AP
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关于此项目
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
33
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
human
technique(s)
direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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General description
人IgG是糖蛋白抗体，含有两条相等的轻链和一对相同的重链。 IgG具有四种不同亚型，范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。经测试证明，抗人IgM（γ-链特异性）-碱性磷酸酶抗体针对人IgA、IgG、IgM、本周蛋白κ和λ骨髓瘤蛋白具有特异性。
Immunogen
纯化人IgG
Application
将从大肠杆菌（ E. coli ）培养物中收集的蛋白质上样至SDS-page凝胶进行蛋白质免疫印迹分析，使用浓度为1:10000的碱性磷酸酶标记山羊抗人IgG（链特异性）作为二抗。使用按1:5000稀释于0.25% BSA/PBS中的碱性磷酸酶标记山羊抗人IgG（γ链特异性）抗体，通过Elisa法检测人血清中的IgG。使用对硝基苯磷酸底物(1mg/ml，Sigma)进行显色。
将从大肠杆菌（ E. coli ）培养物中收集的蛋白质上样至SDS-page凝胶进行蛋白质免疫印迹分析，使用浓度为1:10000的碱性磷酸酶标记山羊抗人IgG（链特异性）作为二抗。使用BCIP/NBT底物(Sigma)，使膜显色。
抗人IgG（γ-链特异性）-碱性磷酸酶抗体适用于ELISA检测（稀释度为1:2500和1:10,000）。该抗体还能用于蛋白质免疫印迹 （1:30,000）和免疫组织化学分析（使用甲醛固定石蜡包埋切片时，稀释度为1:50）。
Physical form
溶剂为0.05 M Tris缓冲液，pH 8.0，含1 mM MgCl2、10 mM甘氨酸、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠
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除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
含少量动物源组分生物产品
此项目有
Ali Akyol et al.
Seizure, 16(3), 233-237 (2007-01-24)
Increased seropositivity for Toxoplasma gondii and Toxocara canis have been observed in epileptic patients. Our aim is to determine whether there is any relationship between these agents and epilepsy in our cryptogenic epilepsy group. We studied specific IgG antibodies against
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D A Heim et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 1(6), 533-544 (2000-08-10)
Host immune responses against foreign transgenes may be a major obstacle to successful gene therapy. To clarify the impact of an immune response to foreign transgene products on the survival of genetically modified cells, we studied the in vivo persistence
Meng-Yun Chou et al.
The Journal of clinical investigation, 119(5), 1335-1349 (2009-04-14)
Atherosclerosis is a chronic inflammatory disease characterized by the accumulation of oxidized lipoproteins and apoptotic cells. Adaptive immune responses to various oxidation-specific epitopes play an important role in atherogenesis. However, accumulating evidence suggests that these epitopes are also recognized by
Assaf Raz et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(18), 4781-4786 (2017-04-22)
The cell wall of Gram-positive bacteria contains abundant surface-exposed carbohydrate molecules that are highly conserved within and often across species. The potential therapeutic usefulness of high-affinity antibodies to cell wall carbohydrates is unquestioned, however obtaining such antibodies is challenging due
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