A3608
牛血清白蛋白 来源于牛血清
lyophilized powder, ≥96% (agarose gel electrophoresis)
别名:
BSA, 牛白蛋白, 牛血清白蛋白
产品名称
牛血清白蛋白 来源于牛血清, lyophilized powder, ≥96% (agarose gel electrophoresis)
biological source
bovine
assay
≥96% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
packaging
poly bottle of
origin
New Zealand origin
technique(s)
immunoprecipitation (IP): suitable
pH
7
~7 (7 % (w/v) in H2O)
solubility
water: slightly soluble
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... ALB(280717)
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Biochem/physiol Actions
Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. High levels of albumin is associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia.
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Preparation Note
来源于新西兰产地的牛血清
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
Application
Bovine Serum Albumin has been used:
- as a component in chromatin immunoprecipitation (ChIP IP) buffer
- in combination with electrospun polystyrene (ESPS) fiber mats for fluorescence microscopy
- as a component of blocking solution and immobilzation studies
- as a component in phosphate buffer saline (PBS)
General description
Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. It belongs to the serum albumins family. It has 17-disulfide bonds. Albumin is the most abundant plasma protein in humans.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
An Interleukin-23-Interleukin-22 Axis Regulates Intestinal Microbial Homeostasis to Protect from Diet-Induced Atherosclerosis
Fatkhullina AR, et al.
Immunity, 49(5), 943-957 (2018)
Micro-chromatin Immunoprecipation (mu ChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
Gillotin S and Guillemot F
Bio-protocol, 6(12), 18-23 (2016)
Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection
Hoy CFO, et al.
Sensing and Bio-Sensing Research, 100304-100304 (2019)
Structural changes during the unfolding of Bovine serum albumin in the presence of urea: A small-angle neutron scattering study
Das A, et al.
Pramana, 63(2), 363-368 (2004)
Bovine serum albumin interactions with metal complexes
Topalua T, et al.
Clujul Medical (1957), 87(4), 215-215 (2014)
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