A3688
抗-小鼠 IgG（全分子）-碱性磷酸酶 山羊抗
affinity isolated antibody, buffered aqueous glycerol solution
别名:
Goat Anti-Mouse IgG (whole molecule)−AP
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关于此项目
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
direct ELISA
dot blot
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
dot blot
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
Species reactivity:
mouse
Citations:
25
Technique(s):
direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
产品名称
抗-小鼠 IgG（全分子）-碱性磷酸酶 山羊抗, affinity isolated antibody, buffered aqueous glycerol solution
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
mouse
should not react with
human
technique(s)
direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
使用碱性磷酸酶偶联的山羊抗小鼠IgG作为次级抗体，在37℃下1小时，通过蛋白质印迹分析人和鼠肿瘤细胞裂解液的各种蛋白表达。
在含有终浓度为0.5M NaCl的TBS/Tween中，使用碱性磷酸酶缀合的山羊抗小鼠IgG（μg/ ml）作为二抗，对支气管肺泡液中的表面活性蛋白A进行检测。
山羊抗小鼠IgG（全分子）-碱性磷酸酶抗体可用于免疫细胞化学分析和ELISA。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析（1篇论文）
蛋白质免疫印迹分析（1篇论文）
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
免疫球蛋白G（IgG）是一种糖蛋白抗体，其可调节免疫反应，如吞噬作用，也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型，即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。
山羊抗小鼠IgG（全分子）-碱性磷酸酶抗体使用IEP与正常小鼠血清和小鼠IgG反应。通过ODD法，抗血清与小鼠IgG1、IgG2a、IgG2b、IgG3、IgA和IgM反应。通过ELISA，偶联物显示与人血清蛋白无反应性。
山羊抗小鼠IgG（全分子）-碱性磷酸酶抗体使用IEP与正常小鼠血清和小鼠IgG反应。通过ODD法，抗血清与小鼠IgG1、IgG2a、IgG2b、IgG3、IgA和IgM反应。通过ELISA，偶联物显示与人血清蛋白无反应性。
Immunogen
纯化小鼠IgG
Other Notes
与人血清蛋白吸附的抗体。
Physical form
在0.05 M Tris（pH 8.0）中的溶液，含1%牛血清白蛋白、10 mM甘氨酸、1 mM MgCl2、50%甘油和15 mM叠氮化钠。
Preparation Note
经过吸附以减少人体样本的背景染色。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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