A3858
牛血清白蛋白 来源于牛血清
lyophilized powder, Protease, essentially free, ≥98% (agarose gel electrophoresis)
别名:
BSA, 牛白蛋白, 牛血清白蛋白
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关于此项目
Biological source:
bovine
Assay:
≥98% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
western blot: suitable
Impurities:
≤5.0% Loss on drying
Concentration:
<0.005 unit/mg solid (Protease)
biological source
bovine
assay
≥98% (agarose gel electrophoresis)
form
lyophilized powder
Quality Level
Gene Information
bovine ... ALB(280717)
purified by
heat shock fractionation
packaging
poly bottle of
origin
New Zealand origin
concentration
<0.005 unit/mg solid (Protease)
technique(s)
western blot: suitable
impurities
≤5.0% Loss on drying
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
Protease, essentially free
storage temp.
2-8°C
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Application
血清白蛋白可用于多种分子生物学应用，例如，作为惰性蛋白稳定剂掺入目标酶和蛋白的储液。 这时的BSA（牛血清白蛋白）不能含蛋白酶，以保持目标蛋白或酶的完整性。
Biochem/physiol Actions
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Preparation Note
源自新西兰血清
热激分级分离法制取
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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